In: General Physiology and Biophysics, vol. 28, no. 3
Danijela Krstić - Mirjana Čolović - Nada Bošnjaković-Pavlović - Anne Spasojević-De Bire - Vesna Vasić
Year, pages: 2009, 302 - 308
The in vitro influence of decameric vanadate species on Na+/K+-ATPase, plasma membrane Ca2+-ATPase (PMCA)-calcium pump and ecto-ATPase activity, using rat synaptic plasma membrane (SPM) as model system was investigated, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. The thermal behaviour of the synthesized decavanadate (V
10) has been studied by differential scanning calorimetry and thermogravimetric analysis, while the type of polyvanadate anion was identified using the IR spectroscopy. The concentration-dependent responses to V 10 of all enzymes were obtained. The half-maximum inhibitory concentration (IC 50) of the enzyme activity was achieved at (4.74 ± 1.15) × 10−7 mol/l for SPM Na+/K+-ATPase, (1.30 ± 0.10) × 10−6 mol/l for commercial Na+/K+-ATPase and (3.13 ± 1.70) × 10−8 mol/l for Ca2+-ATPase, while ecto-ATPase is significantly less sensitive toward V 10 (IC 50 = (1.05 ± 0.10) × 10−4 mol/l) than investigated P-type ATPases. Kinetic analysis showed that V 10 inhibited Na+/K+-ATPase by reducing the maximum enzymatic velocity and apparent affinity for ATP (increasing K m value), implying a mixed mode of interaction between V 10 and P-type ATPases.
How to cite:
Krstić, D., Čolović, M., Bošnjaković-Pavlović, N., Spasojević-De Bire, A., Vasić, V. 2009. Influence of decavanadate on rat synaptic plasma membrane ATPases activity. In General Physiology and Biophysics, vol. 28, no.3, pp. 302-308. 0231-5882.
Krstić, D., Čolović, M., Bošnjaković-Pavlović, N., Spasojević-De Bire, A., Vasić, V. (2009). Influence of decavanadate on rat synaptic plasma membrane ATPases activity. General Physiology and Biophysics, 28(3), 302-308. 0231-5882.