In: NEOPLASMA, vol. 53, no. 3
Cong-Min Gu - You-Kai Zhu - Yi-Hui Ma - Meng Zhang - Bing Liao - Hong-Yang Wu - Han-Liang Lin
Details:
Year, pages: 2006, 206 - 212
About article:
Survivin is the smallest member of mammalian IAP (inhibitor of
apoptosis) family. It is ubiquitous during embryonic development
but is not expressed in normal post-natal tissues, except the
thymus, colonic epithelial cells and CD34+
hematopoietic stem cells. However, its expression is upregulated
during neoplastic transformation in both solid organ and
hematological malignancies, including leukemia and lymphoma. In
this study, we used RNA interference with short hairpin
RNA (shRNA) technique to inhibit survivin expression in a
Burkitt's lymphoma cell line Raji and validated its effects on
apoptosis and cell proliferation.
A survivin-shRNA expression vector were constructed and introduced
into Raji cells. Expression of survivin mRNA and
protein was assessed by RT-PCR and western blot analysis.
Apoptosis index of transfected cells was quantified by flow
cytometry and cell proliferation was enumerated by trypan blue
exclusion.
In Raji cells treated with survivin-shRNA expression vector,
survivin mRNA levels were significantly reduced by
67.14% (transient transfection) and 64.28% (stable transfection)
respectively, compared with control-shRNA treated group
and PBS treated group (p<0.05). The levels of survivin protein
were significantly reduced by 62.50% (transient transfection)
and 60.93% (stable transfection), compared with the two control
groups (p<0.05). Apoptosis index was significantly increased
during transient transfection and stable transfection,
respectively 31.20+/-2.45% and 29.40+/-1.72% (p<0.05).
Survivin-shRNA inhibited the proliferation of Raji cells of stable
transfection.
In conclusion, the vector-based survivin-shRNA can effectively
reduce the expression of survivin gene and induce
apoptosis and growth inhibition of transfected Raji cells. We
suggest that survivin can be regarded as an ideal target for new
anticancer intervention of NHL.
How to cite:
ISO 690:
Gu, C., Zhu, Y., Ma, Y., Zhang, M., Liao, B., Wu, H., Lin, H. 2006. Knockdown of survivin gene by vector-based short hairpin RNA technique induces apoptosis and growth inhibition in Burkitt's lymphoma Raji cell line. In NEOPLASMA, vol. 53, no.3, pp. 206-212. 0028-2685.
APA:
Gu, C., Zhu, Y., Ma, Y., Zhang, M., Liao, B., Wu, H., Lin, H. (2006). Knockdown of survivin gene by vector-based short hairpin RNA technique induces apoptosis and growth inhibition in Burkitt's lymphoma Raji cell line. NEOPLASMA, 53(3), 206-212. 0028-2685.