Some biological actions of PEG-conjugated RNase A oligomers

Year, pages: 2006, 79 - 85

Previously we have shown that monomeric RNase A has no significant biological activity, whereas its oligomers (dimer to tetramer) prepared by lyophilizing from 50% acetic acid solutions, show remarkable aspermatogenic and antitumor activities. Furthermore, conjugates prepared by chemical binding of native RNase A to polyethylene glycol (PEG) have shown a significant aspermatogenic and antitumor activities. In this work we show that the chemical conjugation of PEG to the RNase A C-dimer, and to the two RNase A trimers (NC-trimer and C- trimer) decreases the aspermatogenic activity of the oligomers while increasing their inhibitory activity on the growth of the human UB900518 amelanotic melanoma transplanted in athymic nude mice. Moreover, the PEG-conjugated RNaseA oligomers are devoid, like the free oligomers, of any embryotoxic activity.

Pouckova, P., Skvor, J., Gotte, G., Vottariello, F., Slavik, J., Matousek, J., Laurents, D., Libonati, M., Soucek, J. 2006. Some biological actions of PEG-conjugated RNase A oligomers. In NEOPLASMA, vol. 53, no.1, pp. 79-85. 0028-2685.

Pouckova, P., Skvor, J., Gotte, G., Vottariello, F., Slavik, J., Matousek, J., Laurents, D., Libonati, M., Soucek, J. (2006). Some biological actions of PEG-conjugated RNase A oligomers. NEOPLASMA, 53(1), 79-85. 0028-2685.