MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta

Year, pages: 2014, 103 - 108

Objective. The present study was designed to evaluate whether microRNA-146a, as an NF-кB regulating factor and its adapter proteins (TRAF6 and IRAK1), are affected by diabetes in rat aorta. Methods. Male Wistar rats were randomly divided into control and diabetic groups (n=6 in each). Diabetes was induced by a single injection of streptozotocin (55 mg/kg; i.p.) in 12 h fasted rats. The gene expression of microRNA-146a, NF-κB, IRAK1, and TRAF6 were determined by real time PCR. Results. The expression of microRNA-146a was down-regulated in diabetic aorta when compared with the control group (p<0.05). The mRNA expression levels of NF-кB, TRAF6 and IRAK1 also increased in the diabetic rat aorta when compared with their control counterparts (p<0.01 for all comparisons). Conclusion. These results suggest that down-regulation of microRNA-146a may lead to derangement in NF-кB negative feedback loop, propelling the aorta toward inflammation.

Emadi, S., Soufi, F., Khamaneh, A., Alipour, M. 2014. MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta. In ENDOCRINE REGULATIONS, vol. 48, no.2, pp. 103-108. 1210-0668. DOI: https://doi.org/10.4149/endo_2014_02_103

Emadi, S., Soufi, F., Khamaneh, A., Alipour, M. (2014). MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta. ENDOCRINE REGULATIONS, 48(2), 103-108. 1210-0668. DOI: https://doi.org/10.4149/endo_2014_02_103

Publisher: AEPress, s.r.o.