In: NEOPLASMA, vol. 51, no. 5
V. Habalová - J. Šalagovič - I. Kalina - J. Štubňa
Details:
Year, pages: 2004, 352 - 357
About article:
Many genes involved in the metabolism of carcinogens have been
found to be polymorphic in the human population, and specific
alleles are associated with increased risk of cancer at various
sites. The etiology of most commonly occurring cancers cannot be
explained by allelic variability at a single locus. A combined
analysis of two polymorphic enzymes, glutathione S-transferase M1
(GSTM1), microsomal epoxide hydrolase (EPHX1)) and their
implication as lung cancer risk factors was performed in a case-
control study of non small cell lung cancer. Polymerase chain
reaction (PCR) or PCR-RFLP-based methods were used to detect
variant genotypes of GSTM1 and EPHX1 (113Tyr-113His in exon 3 and
139His-139Arg in exon 4) in 150 controls and group of lung cancer
patients (n=121). The slow 113His EPHX1 allele tended to be more
frequent among the patients (frequency 0.587) than among the
controls (0.320) (Fisher´s exact test, p=0.33). The combined
EPHX1 homozygote genotype His113/His139 (predicted very slow
activity) versus all other genotype combination was associated
with an increased risk of lung cancer (OR=2.29; 95% C.I.=0.94-
5.82), particularly in non-smokers (OR=11.23; 95% C.I.=1.48-
88.41). Polymorphism in GSTM1 had no statistically significant
impact on lung cancer risk alone (OR=1.09; 95% C.I.: 0.65-1.82).
However, obtained the results revealed that combinations GSTM1
null with homozygote His113/His139 genotype (predicted very slow
activity EPHX1) significantly increased lung cancer risk (OR=3.65;
95% C.I.: 1.04-16.07). No overall relationship between genotype
combinations predicted high EPHX1 activity and lung cancer risk
was confirmed in all followed respects. However, the number of
investigated individuals in our study was relatively small,
therefore these findings should be judged with circumspectness.
How to cite:
ISO 690:
Habalová, V., Šalagovič, J., Kalina, I., Štubňa, J. 2004. Combined analysis of polymorphisms in glutathione S-transferase M1
and microsomal epoxide hydrolase in lung cancer patients. In NEOPLASMA, vol. 51, no.5, pp. 352-357. 0028-2685.
APA:
Habalová, V., Šalagovič, J., Kalina, I., Štubňa, J. (2004). Combined analysis of polymorphisms in glutathione S-transferase M1
and microsomal epoxide hydrolase in lung cancer patients. NEOPLASMA, 51(5), 352-357. 0028-2685.