Institute of Chemistry
Topic
Biochemical characterization of inclusion bodies of enzymes required for hyaluronic acid synthesis
PhD. program
Biochemistry
Name of the supervisor
Ing. Jozef Nahálka, PhD.
Contact:
Receiving school
Faculty of Natural Sciences, Commenius University Bratislava
Annotation
Historically, the immobilization of enzymes has been introduced for their reuse in various industrial biotransformations, for more economical use of the biocatalyst. Usually, the enzymes are adsorbed on a solid support or entrapped in gel particles. In the last decade, recombinant enzyme production technologies have made it possible to combine process of enzyme production and immobilization directly in the cell, which we now refer to as "in vivo" enzyme immobilization. One alternative to "in vivo" immobilization is pull-down of recombinant protein production into active inclusion bodies. The dissertation will focus on the biochemical characterization of inclusion bodies of enzymes required for the synthesis of hyaluronic acid. It will be preparation and characterization of HAS (Hyaluronan synthetase), NahK (N-acetylhexosamine 1-kinase), GlmU (GlcNAc-1-P uridyltransferase), GlcAK (GlcA 1-kinase) and USP (UDP-sugar pyrophosphorylase).