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PhD. Topics

Institute of Chemistry

Mannanolytic acetyl esterases
PhD. program
Name of the supervisor
Mgr. Vladimír Puchart, PhD.
Receiving school
Faculty of Chemical and Food Technology, Slovak University of Technology
Galactoglucomannan is the most common hemicellulose of softwoods and the second most common hemicellulose of hardwoods. Acetylation of its backbone, which significantly complicates its enzymatic depolymerization, also contributes to the heterogeneity of this polysaccharide. Despite these findings, almost nothing is known about acetylmannanesterases, as only a few have been isolated and even those that have been isolated have not been further characterized. This topic will undoubtedly bring completely new knowledge, which will certainly contribute not only to a better characterization of other mannanolytic enzymes, but also to their wider practical use.
Aims: 1. To study substrate specificity and regiospecificity of various hemicellulolytic as well as non-hemicellulolytic acetyl esterases on acetylated mannans; 2. Looking for new enzymes deesterifying plant mannans; 3. To establish the enzymes’ role in the decomposition of this hemicellulose, in particular taking into account their cooperation with other mannanolytic enzymes