Facebook Instagram Twitter RSS Feed PodBean Back to top on side

PhD. Topics

Polymer Institute

Implantable polymeric devices using non-covalent and covalent click chemistry with self-healing characteristics for cell encapsulation
PhD. program
Organic chemistry
Name of the supervisor
Abolfazl Heydari, PhD.
Receiving school
Faculty of Natural Sciences, Comenius University
The Ph.D. topic deals with the in-situ preparation of hydrogels based on polysaccharides stabilized by both transient non-covalent and covalent cross-links within its network structure. Resulting hydrogels as implantable polymeric devices should be suitable predominantly for the long-term immunoprotection of transplanted cells. This dual cross-linking strategy will be established to overcome the low mechanical and chemical stability of ionically cross-linked cell-laden hydrogels upon exposure to the in vivo environment. In this Ph.D. thesis, a clickable polysaccharide, having enough polyelectrolyte-binding sites and biocompatible moieties, will be synthesized by introducing the ionic-binding and click-reacting functional groups on the backbone of modified polysaccharides. The obtained polymers will lead to the formation of hydrogels via combining the bioorthogonal click-non-covalent and click-covalent crosslinks. The host-guest complexation will be selected as a click-non-covalent bond. The click-covalent bond will include “copper- and light free” reactions such as thiol/aza-Michael addition and Diels-Alder cycloaddition. Finally, the physicochemical properties of the achieved hydrogels and their cytocompatibility behavior will be studied by encapsulation of various cell types (e.g. insulin-producing β-cell, native islets) with the potential for the in vivo testing.