Biomedical Research Center SAS
Topic
Study of the effect of pathogenic mutations on the allosteric regulation of the ryanodine receptor using molecular simulation
PhD. program
biophysics
Name of the supervisor
Ing. Miloslav Karhánek, PhD.
Contact:
Receiving school
Faculty of Science, Pavol Jozef Šafárik University
Annotation
Ryanodine receptors (RyR) are ion channels responsible for control of excitation-contraction coupling in skeletal (RyR1) as well as cardiac muscle (RyR2). Pathogenic RyR mutations lead to dysregulation of channel activity by Ca2+ and Mg2+ ions, causing arrhythmias and malignant hyperthermia. The aim is to assess the effect of selected pathogenic mutations on the allosteric pathways between the binding sites of regulatory ligands (Ca2+, Mg2+, ATP, xanthines) and the gating mechanism in the RyR pore, using molecular simulation.
Methods: structural biology; programs NAMD / GROMACS
Methods: structural biology; programs NAMD / GROMACS