Institute of Molecular Biology
Water/Wastewater epidemiology: Development of robust and reliable molecular detection systems for surveillance of disease outbreaks
Epidemiológia vody/odpadovej vody: Vývoj spoľahlivých molekulárno-biologických detekčných metód pre dohľad nad ohniskami epidémií
| Duration: | 1.1.2021 - 31.12.2023 |
| Program: | Bilateral - other |
| Project leader: | Dr. Pangallo Domenico DrSc. |
| Annotation: | Before being a health emergency, the COVID-19 pandemic should be considered as a problem related to the unsustainable exploitation of the environment. We retain that the monitoring of the environmental microorganisms and viruses can be a suitable solution to face the next pandemics. Early warning systems are necessary to assess the health status of our cities using the samples recovered from sewage, wastewater, and aquatic environments. These systems can be based on reliable and quick molecular approaches that consent to make prompt decisions against the spreading of dangerous outbreaks. In our project, these molecular systems will be targeted to antibiotic-resistant bacteria (ARB), Dengue virus (DENV), and SARS-CoV-2. ARB are already a real threat, and many pandemics that have not made so much clamor in media occur periodically. DENV is another dramatic reality in many countries (including Taiwan) of tropical and subtropical regions, but by climate change and globalization, this threat can readily spread to other world regions. SARS-CoV-2 is new, and unfortunately, no therapy and vaccine are available at the moment. Through the cooperative works, we will develop and optimize several molecular approaches based on qPCR and RT-qPCR techniques. Such PCR assays will reveal and quantify the described pathogens, and they will be coupled with internal amplification controls able to guarantee their efficiency. Our investigation will be completed by the in-depth analysis of viral and microbial communities, including the resistome. This analysis will consent better to understand the viral and bacterial interactions in studied environments. For this purpose, two different high-throughput sequencing platforms, MinION and NovaSeq 6000, will be used. |
Use of honeybees, honey and other apiary products for biomonitoring of low-level radioactive phosphogypsum stacks
Použitie včiel, medu a ostatných včelích produktov na biomonitorovanie nízko rádioaktívneho fosfosadrovcového odpadu
| Duration: | 1.5.2023 - 30.4.2025 |
| Program: | Other |
| Project leader: | Ing. Majtán Juraj DrSc., MBA, FIFST |
The total number of projects: 2