Biomedical Research Center SAS
Topic
Subtyping and detection of herpesvirus mutations using PCR and sequencing
PhD. program
microbiology and virology
Year of admission
2026
Name of the supervisor
RNDr. Katarína Lopušná, PhD.
Contact:
Receiving school
Faculty of Natural Sciences, Comenius University in Bratislava
Annotation
Patients with tumors suffer from immunodeficiencies that impair the function of one or more components of the immune system. Viral infections pose a particularly high risk in this regard. The proposed topic focuses on the differential diagnosis of non-specifically identifiable herpesviruses in patient samples, as well as the identification of mutations associated with antiviral resistance. The aim of the proposed project is:
1. detection of HCMV, HHV-6, and EBV from blood
samples and fingernails of patients using PCR and quantitative PCR (qPCR)
2. identification of HCMV, HHV-6, and EBV mutations that could be associated with resistance
3. experimental confirmation of newly identified mutations using Sanger sequencing.
1. detection of HCMV, HHV-6, and EBV from blood
samples and fingernails of patients using PCR and quantitative PCR (qPCR)
2. identification of HCMV, HHV-6, and EBV mutations that could be associated with resistance
3. experimental confirmation of newly identified mutations using Sanger sequencing.