Centre of Biosciences SAS
Topic
Effects of low-frequency ultrasound in wounds treatment tested in Japanese quail CAM model
PhD. program
Animal Physiology
Year of admission
2025
Name of the supervisor
Mgr. Mariana Máčajová, PhD.
Contact:
Receiving school
Faculty of Natural Sciences of Comenius University in Bratislava
Annotation
Currently, the treatment of infected wounds is also a major challenge in medicine. At a time of increasing antibiotic resistance, alternative therapies are also coming into their own. Sonodynamic therapy is an emerging therapeutic procedure combining ultrasound and a chemical agent - a sonosensitiser. After its activation by ultrasound, reactive oxygen molecules (ROS) are produced in the tissue and have a cytotoxic effect on the surrounding cells. Sonodynamic therapy has potential applications in the treatment of cancer, superficial bacterial and yeast infections, and other pathological conditions. The aim of this dissertation will be to establish an in vivo model for the investigation and treatment of superficial infections. We will apply sonodynamic therapy using low frequency ultrasound to a quail chorioallantoic membrane (CAM) model that will be infected with bacteria and yeast. After therapy, we will monitor angiogenic changes in CAM tissue, expression of selected genes, as well as histological and immunohistochemical changes.