Institute of Chemistry
EXosome Analysis Complementary To Diagnostic eXaminations
Analýza exozómov ako komplementárna diagnostická metóda
| Duration: |
1.9.2025 - 31.8.2028 |
| Program: |
SRDA |
| Project leader: |
Ing. Bertók Tomáš DrSc., MBA |
| Annotation: |
Cancer is one of the leading causes of deaths globally, with annual incidence of 20.0M in 2022. Moreover, the burden is estimated to grow even further to 32.6M in 2045 (Globocan data). The tumor biopsy increases the cost of patient care and in some cases the turnaround time for getting results, thus novel approaches have been developed using body fluids – so-called liquid biopsy. Among autoimmune diseases, rheumatoid arthritis is one of the most common ones with the incidence of about 150,000 to 200,000 new cases in Europe only. As well as in cancer progression, glycans play an important role in defining anti- or proinflammatory role of antibodies. So-called seronegative arthritis, a category of inflammatory joint conditions without detectable autoantibodies comprises about 15% of all patients diagnosed with rheumatoid arthritis. Since ~70+% of all diagnoses rely on laboratory tests results (according to CDC), the importance of implementing novel biomarkers is a high unmet medical need. In our previous work, we´ve developed bioanalytical devices with low detection limits for analyzing glycoproteins down to femtomolar levels and applied these platforms for the analysis of real human samples using nanostructured interfaces resistive to non-specific interactions. In this project, we will focus on the application of these findings in the area of exosome analysis, which are harder to analyze due to their heterogenic nature and relatively low stability. The ultimate goal in the glycomic analyses is the possibility to glycoprofile biomarkers of choice omitting the “heavy machinery” approach (LC-MS), but rather to extract the most crucial information about the presence/absence of cancer-associated glycan epitopes promoting cell aggressiveness and metastases in an easy-to-use, inexpensive way using a simple, high-throughput table-top platforms, such as lectin-based immunoassays. |
Mapping the Glycome and Glycosylated Proteins in Alzheimer's disease
Mapovanie glykómu a glykozylácie proteínov spojených s Alzheimerovou chorobu
| Duration: |
1.9.2025 - 31.8.2028 |
| Program: |
SRDA |
| Project leader: |
Ing. Ďatková Anna PhD. |
| Annotation: | Nowadays, there are 55 million people with dementia worldwide, and with the aging of the population, a sharp increase in the incidence of this disease is expected. Early diagnosis is a key aspect in the treatment of any disease. Current diagnosis of Alzheimer disease (AD) is made by analysis of proteins/peptides in cerebrospinal fluid (CSF) or blood. AD can also be diagnosed using brain imaging techniques, but such techniques are expensive and can only be performed in specialized hospitals with trained personnel. Many studies suggest that changes in glycosylation may play a key role in the pathogenesis and progression of the disease. The presented project focuses on tracking changes in glycome and glycosylation of selected glycoproteins associated with AD. The main goal of the project is to identify changes in the glycoprofile of various samples, altered glycosylation of proteins and to use this knowledge to improve AD diagnostic methods. The project methodology includes advanced glycosylation techniques, glycomics analysis and bioinformatics tools for data analysis and interpretation. We expect that the results of this project will contribute to a better understanding of the molecular mechanisms in AD and open new possibilities for diagnosis and potential treatment for such a disease. The importance of the project lies in its potential to identify new biomarkers for the early diagnosis of AD, which could fundamentally improve the prognosis and quality of life of patients. |
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Antibakteriálne katiónové polysacharidy proti rezistentným nozokomiálnym patogénom - štúdium vzťahu štruktúry a biologických vlastností
Antiviral drugs against COVID-19: Design, synthesis and biological activity testing of specific inhibitors of viral proteases of coronavirus SARS-CoV-2
Antivirálne liečivá proti COVID-19: Dizajn, syntéza a testovanie aktivity špecifických inhibítorov virálnych proteáz koronavírusu SARS-CoV-2
Baeyer-Villiger oxidations, immobilized cells, mechanically stirred bioreactors, pneumatically stirred bioreactors, mathematical modeling
Baeyer-Villigerove oxidácie, imobilizované bunky, mechanicky miešané bioreaktory, pneumaticky miešané bioreaktory, matematické modelovanie
Bioinspired oxidative coupling of natural phenols
Bioinšpirovaný oxidačný kapling prírodných fenolov
Biosensoring devices based on functionalized micro- and nanoscale biointerfaces for glycomic analyses
Biosenzorické zariadenia založené na funkcionalizovaných mikro- a nanoškálovaných biorozhraniach pre glykomické analýzy
Detailed analysis and elucidation of functions of Cka1 and Ksg1 protein kinases using the conditional ATP analog-sensitive mutants
Detailná analýza a objasnenie funkcie Cka1 a Ksg1 proteínkináz využitím ich kondičných na ATP analógy citlivých mutantov
Design, synthesis and biochemical evaluation of Golgi α-mannosidase II inhibitors
Dizajn, syntéza a biochemické testovanie selektívnych inhibítorov Golgiho α-manozidázy II
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Glykozidhydrolázy rodín 16,28 a 72 - ich charakterizácia, štruktúra a funkcia v bunkových stenách rastlín a húb
In vivo enzyme immobilization applied in the synthesis of hyaluronic acid
In vivo imobilizácia enzýmov aplikovaná v syntéze kyseliny hyalurónovej
Innovative 2D Nanomaterial-Enhanced Biosensors And Enzymatic Platforms: The Future of Specialty Chemical Production
Inovatívne 2D nanomateriálmi vylepšené biosenzory a enzymatické platformy: Budúcnosť výroby špeciálnych chemikálií
| Duration: |
1.1.2026 - 31.12.2029 |
| Program: |
VEGA |
| Project leader: |
Ing. Hrončeková Štefánia PhD. |
| Annotation: | This project focuses on developing goldene, an innovative nanomaterial, and other 2D nanomaterials such as MXenes for the design of ultrasensitive electrochemical nanobiosensors. These will enable advanced analysis of multienzyme cascade reactions for producing specialty chemicals, particularly epsilon-caprolactone. Compared to conventional methods, the new biosensors will offer faster monitoring, flexible process control, and reduced solvent consumption. The project includes goldene synthesis, electrochemical studies, biosensor development and bioelectrochemical testing, followed by validation on real biotransformation samples. Research will also continue on epsilon-caprolactone production using both free and immobilised recombinant E. coli BL21 (DE3) cells with co-expressed enzyme cascades. Integrating novel 2D nanomaterials, advanced electrochIntegrating novel 2D nanomaterials, advanced electrochemical/characterisation methods, bioreactor engineering, and immobilisation will optimise biosensor performance. |
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Inovované imobilizované biokatalyzátory na báze Baeyer-Villigerových monooxygenáz, enzýmových kaskád a hydroláz epoxidov pre ekologickú prípravu chemických specialít
Joint theoretical and experimental approaches to address modern challenges of molecular biology.
Integrácia teoretických a experimentálnych prístupov pri riešení moderných výziev molekulárnej biológie.
Interaction of saccharide epitopes of epidemiologically interesting Candida species with C-type lectins
Interakcie sacharidovývh epitopov epidemiologicky zaujímavých druhov Candida s lektínmi typu C
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Izolácia, purifikácia, charakterizácia a využitie diglykozidáz
Cationic polysaccharides as potential antibacterial agents for direct and combination therapies of infections caused by resistant pathogens
Katiónové polysacharidy ako potenciálne antibakteriálne agensy pre priamu a kombinovanú terapiu infekcií spôsobených rezistentnými patogénmi
Phylloplane and pedobiont yeasts as beneficial agens for plants
Kvasinky z fylosféry a pôdy ako užitočné agensy pre rastliny
Microscopic cyanobacteria and algae as an important source of exopolysaccharides
Mikroskopické sinice a riasy ako významný zdroj exopolysacharidov
Modification of surfaces as barrier to protein adsorption
Modifikácia povrchov ako bariéra pre adsorpciu proteínov
Insight into the immunobiological properties of Candida albicans extracellular vesicles (EVs) cargo with a focus on active carbohydrate molecules important for host-pathogen interaction
Nahliadnutie do imunobiologických vlastností obsahu extracelulárnychvezikúl (EVs) Candidaalbicans so zameraním na aktívne molekuly sacharidov dôležité pre interakciu hostiteľ-patogén
Uncharted Glycosylation Complexity: Investigation of its Dynamic Mechanisms and Disease-Relevant Glycobiomarkers
Nezmapovaná komplexnosť glykozylácie: Výskum jej dynamických mechanizmov a glykobiomarkerov relevantných pre ochorenia
Novel enzymes for the decomposition of the most recalcitrant epitopes of plant polysaccharides
Nové enzýmy pre rozklad najneprístupnejších epitopov rastlinných polysacharidov
| Duration: |
1.1.2022 - 31.12.2025 |
| Program: |
VEGA |
| Project leader: |
Mgr. Puchart Vladimír PhD. |
| Annotation: | This project is a continuation of long-term successful effort of the authors’ group to expand current knowledge on
microbial enzymes involved in plant cell wall degradation. The project is a part of a world-wide effort to develop
processes for a complete exploitation of this annually renewable source of carbon and energy.. The project will
study substrate specificity and regioselectivity of novel, poorly examined xylanases as well as auxiliary xylanolytic
enzymes, specifically, beta-xylosidases, alpha-glucuronidases, alpha-L-arabinofuranosidases, and acetylxylan
esterases, and relate them to their structural diversity. The project will be focused on the degradation of the most
recalcitrant epitopes originating from xylans of various types as the most abundant plant hemicellulose. The
unraveling of so far unknown action will contribute to a development of efficient and environment-friendly
industrial processes of biodegradation and bioconversion of plant biomass and its conversion to beneficial
products. |
Novel yeast enzymes for bioconversion of plant biomass
Nové kvasinkové enzýmy na biokonverziu rastlinnej biomasy
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Nové prístupy na zlepšenie microarray detekčnej technológie pre vysokovýkonnú glykotypizáciu
Advanced 2D nanoscaled "MXene" interfaces as perspective immobilisation platforms for design of (bio)sensors for cancer biomarkers detection and glycan enrichment
Pokročilé 2D nanorozmerné "MXénové" rozhrania ako perspektívne imobilizačné platformy pre návrh (bio)senzorov na detekciu biomarkerov rakoviny a obohatenie glykánov
Origin and function of novel meningeal subpopulation of Lyve1 macrophages in health and disease
Pôvod a funkcia nových meningeálnych Lyve1 pozitívnych makrofágov v zdraví a chorobe
Progressive Approaches in Prostate Cancer Diagnosis Through the Integration of Advanced Techniques - Magnetic Separation and FRET Detection
Progresívne prístupy v diagnostike rakoviny prostaty prostredníctvom prepojenia pokročilých techník - magnetickej separácie a detekcie FRET
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Purifikácia a kryštalizácia nešpecifickej xyloglukán endotransglykozylázy
Synthesis of nanocomposites of transition metal oxides, study of the influence of plasma on their photocatalytic efficiency with application in wastewater treatment
Syntéza nanokompozidov oxidov prechodných kovov, štúdium vplyvu plazmy na ich fotokatalytickú účinnosť s uplatnením pri čistení odpadových vôd
Synthesis, physicochemical, biological properties of glycoconjugates, N-heterocycle-based precursors and polysaccharide derivatives as potential anticancer and antiviral agents
Syntéza, fyzikálno-chemické a biologické vlastnosti prekurzorov na báze glykokonjugátov, N-heterocyklov a derivátov polysacharidovako potenciálnych antikarcinogénnych a antivirotických liečiv
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Štipendiá pre excelentných PhD. študentov a študentky (R1) / Vývoj a aplikácia moderných metód pre detekciu glykánových biomarkerov
The effect of pathologic mutations and post-translational modifications onthe functions of mitochondrial pocessing peptidase essential for transport to mitochondria
Účinok patologických mutácií a posttranslačných modifikácií na funkcie mitochondriálnej procesujúcej peptidázy nevyhnutnej pre transport do mitochondrií
Investigation of substances and factors with a potential effect on the pathogenicity of various strains of bacterium Paenibacillus larvae causing American foulbrood
Výskum látok a faktorov s potenciálnym vplyvom na patogenicitu rôznych kmeňov baktérie Paenibacillus larvae spôsobujúcej mor včelieho plodu
The utilisation of indigenous microorganisms and natural compounds for improvement of seed germination, plant growth and the amelioration of the abiotic stress
Využitie pôvodných mikroorganizmov a prírodných zlúčenín na zlepšenie klíčenia, rastu rastlín a na zmiernenie abiotického stresu
Golden(e) approach: Next-generation of 2D nanomaterial-enabled advanced biosensors and immobilised systems using enzymes and viable whole cells with enzyme cascades for use in production of chemical specialties
Zlatý prístup: Nová generácia 2D pokročilých biosenzorov s podporou nanomateriálov a imobilizované systémy využívajúce enzýmy a viabilné celé bunky s enzýmovými kaskádami na produkciu chemických špecialít
| Duration: |
1.9.2025 - 31.8.2029 |
| Program: |
SRDA |
| Project leader: |
Ing. Hrončeková Štefánia PhD. |
| Annotation: | The project solution is aimed at obtaining new knowledge from the point of view of the preparation of the innovative nanomaterial goldene, the use of nanomaterials based on MXenes and others for the design of ultrasensitive amperometric biosensors and their use for advanced analysis of multienzyme cascade reactions for the production of chemical specialties. The use of new biosensors will enable faster monitoring, more flexible management of cascade reactions compared to the conventional analytical procedures and potential and environmentally beneficial reduction in the consumption of harmful solvents. Biofunctionalisation of the biosensor surface with a high density of cells/cofactors/enzymes will ensure high sensitivity of the developed biosensors. Characterisation of step-by- step development of biosensors and testing of their bioelectrochemical activity will precede validation using real samples from biotransformations. Part of the project will be the continuation of research and development of new immobilisation procedures of recombinant E. coli cells with co-expressed enzyme cascades as well as individual enzymes of the cascades. When solving the project, bioreactor engineering methods, advanced biopolymer materials and the most modern photonics methods will be used in cooperation with top partners in order to characterise and set optimal biocatalytic parameters of cascade reactions for the model production of chemical specialties. |
The total number of projects: 36
