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Institute: Institute of Neurobiology

Investigation of potential and role of the central canal lining in the reaction to the spinal cord injury
Analýza potenciálu a úlohy výstelky centrálneho kanála pri regenerácii miechy po poranení
Program: SRDA
Project leader: RNDr. Račeková Enikö CSc.
Duration: 1.7.2016 - 30.6.2020

The application of combined therapy to suppress secondary damage after spinal cord trauma
Aplikácia kombinovanej terapie na potlačenie sekundárneho poškodenia miechy po traume
Program: SRDA
Project leader: RNDr. Lukáčová Nadežda DrSc.
Annotation:Despite progress made in the understanding of the pathogenesis of traumatic spinal cord injury in humans, and in improvements of its early diagnostics, we still do not have effective treatment. Mechanisms of secondary injury, as a consequence of primary injury, represent extensive windows for therapeutic manipulations by appropriate exogenous and endogenous interventions. We intend to promote axon regeneration and functional recovery after SCI by blocking the signaling pathways for axonal growth inhibitors in combination with therapeutic effect of the biodegradable material, gradually releasing neurotrophic factors. Because the growth of axons into a spinal cord lesion can be disorganized, we suppose, that long term application of weak electrical field (ES) over injurz site can support their arrangement in linear alignment present in the intact spinal cord. In addition, our objective is to guide the axonal alignment, by AAV9 - GDNF vector, applied subpialy in the vicinity of traumatic lesion. We suppose, that the use of adeno-associated viral (AAV) vector allow long-term and stable transgene expression of GDNF and in combination with ES can be promising intervention for neurogenesis and remyelination, and functional recovery.
Duration: 1.7.2016 - 30.6.2020

Localization and role of endothelial nitric oxide synthase in the neurogenic region of the rat during postnatal period
Lokalizácia a úloha endotelovej syntetázy oxidu dusnatého v neurogénnej oblasti potkana v postnatálnom období
Program: VEGA
Project leader: RNDr. Martončíková Marcela PhD.
Duration: 1.1.2017 - 31.12.2019

Neuroprotection in the process of ischemic tolerance acquisition from the perspective of rat brain pathways monitoring (proteomic MALDI-TOF/TOF study)
Neuroprotekcia v procese získania ischemickej tolerancie z pohľadu sledovania reakčných dráh v mozgu potkana (proteomická MALDI-TOF/TOF štúdia)
Program: VEGA
Project leader: MVDr. Némethová Miroslava PhD.
Annotation:Project is devoted to the global brain ischemia study, mainly to processes and mechanisms involved in the pathophysiology of CNS ischemic injury. Targeted examining of neuroprotective mechanisms and finding possibilities of brain cells protection after ischemia are primary topics of the project. It is known that neurons have a natural ability to tolerate damage caused by ischemic episode. A conditioning or second stress seems to be starting condition of this process, if used prior to ischemia or during postischemic reperfusion interval within two days after ischemia. This procedure is able to prevent the occurrence of delayed neuronal death. Implementation of the objectives of the project will be based on proteomic analysis of ischemic and tolerant brain tissue by mass spectrometry. Identification of differentially expressed proteins along with their classification into metabolic and signaling pathways will allow us to monitor the mechanisms involved in the targeted protection and survival of neurons.
Duration: 1.1.2018 - 31.12.2020

Neuroprotective mechanisms of the AT2 receptor stimulation after traumatic spinal cord injury.
Neuroprotektívne mechanizmy zahrnuté v stimulácii AT2 receptora po traumatickom poškodení miechy.
Program: VEGA
Project leader: RNDr. Pavel Jaroslav PhD.
Annotation:There is a prolonged social as well as medical demand on intensive research, because no effective and trustworthy clinical treatment for patients with the spinal cord injury (SCI). Since most of the physiological and pathological effects of the Angiotensin II are mediated through the AT1 receptor stimulation, the AT2 receptor study was until recently at the periphery of scientific interest. At present, the AT2 receptor represents a potentially important area of investigation with possible therapeutic application since it was shown its wide neuroprotective potential. Our main objective is to contribute to the elucidation of the neuroprotective mechanisms in which the AT2 receptor plays an important role.
Duration: 1.1.2016 - 31.12.2018

Axonal regeneration in biosynthetic nerve guide conduits.
Regenerácia nervových vlákien v biosyntetických vodičoch.
Program: SRDA
Project leader: MVDr. Vanický Ivo CSc.
Annotation:In adult mammals, nerve fibers are capable of regeneration. In peripheral nerve after injury, axons from the proximal stump regrow across the site of injury into the distal stump and create new connections with their target organs. Current surgical techniques allow for a direct reconstruction of the severed nerve (end-to-end) only if the missing segment is not too large. After devastating injuries, autologous nerve grafts can be used. Synthetic nerve guides for nerve regeneration can be used as a substitute for autologous grafts. Regrowth of regenerating fibers across the synthetic nerve guides is limited to relatively short distances. This restriction is surprising, because in the distal stumps, regenerating axons are able to grow over long distances. Advances in the synthesis of biocompatible polymers provide new biomaterials with optimized properties. In the present project, we plan to use biosynthetic guides made from new elastomeric materials. The properties of the nerve guide will be modified and tested in vitro, with the goal to stimulate regeneration over long distances. The effectiveness of these modifications on regeneration process will be tested in vivo. 15 Žiadateľská organizácia Neurobiologický ústav SAV Co-ordinating organization Slovak Academy of Sciences, Institute of Neurobiology
Duration: 1.7.2015 - 30.6.2019

The therapeutic effects of stem cells conditioned medium on the repair of the spinal cord damaged tissue: a comparative ex vivo study.
Terapeutické účinky kondiciovaného média kmeňových buniek na reparáciu poškodeného tkaniva miechy: porovnávacia ex vivo štúdia.
Program: VEGA
Project leader: RNDr. Slovinská Lucia PhD.
Annotation:Spinal cord injury is a neurodegenerative disease of the CNS, which leads to damage and death of the spinal cord cell population, namely neurons, oligodendrocytes, astrocytes. Cell therapy is a key area of regenerative medicine to find suitable sources of transplanted cells or their products, allowing restoration of damaged tissue. The aim of the present project is to establish an ex vivo model of traumatic injured CNS tissue in the form of organotypic spinal cord slices. Using this model we will investigate the effects of the conditioned medium of the stem cells (mesenchymal stem cells from bone marrow and adipose tissue, neural progenitors) on the repair of damaged rat spinal cord tissue. We compare the effectiveness of different conditioned media on the base of survival and proliferative potential of glial cell population, neurons and nerve fibers overgrowth. We suppose, that the ex vivo model of mechanical damage will be a good method for the study of potential therapeutic strategies in CNS damage.
Duration: 1.1.2016 - 31.12.2018

The effect of GDNF vector and block of inhibition molecules on interneuronal connections and axonal outgrowth after cervical and thoracic spinal cord injury
Účinok GDNF vektora a blokovania inhibičných molekúl na interneuronálne prepojenia a prerastanie axónov po cervikálnom a torakálnom poškodení miechy
Program: VEGA
Project leader: RNDr. Lukáčová Nadežda DrSc.
Annotation:The aim of proposed project is 1) to examine molecular changes in the respiratory nuclei and interneurons, and reinervation of bulsbospinal pathway after cervical compression and application of AAV9-GDNF vector that allow long-term and stable transgene expression of GDNF in the vicinity of the SCI lesion, 2) to promote axonal regeneration and to improve functional recovery after thoracic compression by blocking the signaling pathways for axonal growth inhibitors. We will compare two ways (intraparenchymal and subpial) of application, and in combination with post-traumatic rehabilitation training we will determine, which interneuronal connections (commissural, propriospinal, reticulospinal) promote functional outcome.
Duration: 1.1.2017 - 31.12.2019

The total number of projects: 8