The list of national projects SAS

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Institute: Plant Science and Biodiversity Center

Looking for suitable genetic markers for species recognison in Russula subgenus Polychromidia
Hľadanie genetických markerov pre rozlíšenie druhov v rámci Russula podrodu Polychromidia
Program: Inter-academic agreement
Project leader: Mgr. Adamčík Slavomír PhD.
Duration: 1.1.2016 - 31.12.2017

The quest for tolerant varieties - Phenotyping at plant and cellular level
Hľadanie tolerantných odrôd - fenotypovanie na úrovni rastliny a bunky
Program: COST
Project leader: RNDr. Salaj Terézia DrSc.
Annotation:The project is focused on the study of physiological, biochemical and structural aspects of somatic embryogenesis. Somatic embryogenesis is development of embryos from somatic cells of a plant body under in vitro conditions. The process involves several steps as initiation of embryogenic tissues, maturation and germination of somatic embryos and transfer to soil. Each of the mentioned steps will be studied in details with aim to improve the process. The role of nutrient medium composition and explants will be tested for the initiation.The structure of somatic embryos will be followed by light microscopy.For maintenance of embryogenic tissues solid or liquid media will be used as well as for their long-term cultivation the method of cryopreservation will be tested. The maturation of somatic embryos is a very important step resulting in the whole plant (somatic seedlings) regeneration. The project focuses also on the optimization of maturation process and plantlet regeneration. An important part of the project is the study of embryogenic tissues and comparison to nonembryogenic callus culture by analysis using proteomic methods to get deeper insight to the process of somatic embryogenesis.
Duration: 22.5.2014 - 21.5.2018

Chitinases and AGPs as proteins diversifying genotypes with high ability to microspore embryogenesis from non-embryogenic in crops
Chitinázy a AGPs ako proteíny diverzifikujúce genotypy s vysokou schopnosťou mikrospórovej embryogenézy z ne-embryogénnych plodín
Program: Bilateral - other
Project leader: Ing. Moravčíková Jana PhD.
Annotation:The project is aimed at linking of the two research laboratories with expertise on embryogenesis (Poland) and molecular analysis (Slovakia). It is dedicated to the study and optimization of androgenesis for rye as a tool for more efficient breeding through dihaploid lines. On biological materials with contrasting androgenic potential it will be evaluated the effect of action of abiotic factors such as cold and osmotic stress. It will be studied in detail the changes in accumulation of chitinase enzymes and arabinogalactans, which in model plants had a beneficial effect on androgenesis. Results of the project are intended to impart the knowledge about the process of androgenesis and factors affecting its efficacy in rye.
Duration: 1.1.2016 - 31.12.2017

Non-native tree species for european forests - experiences, risks and opportunities
Nepôvodné druhy drevín pre európske lesy - skúsenosti, riziká a možnosti
Program: COST
Project leader: RNDr. Kormuťák Andrej DrSc.
Annotation:The management of tree species non-native to European geographical regions has a long tradition within forestry management practice. Their introduction to Europe (initially focussed on growing tree species) dates back to the 18th century when enormous demands were being made on natural resources to sustain the on-going industrialization of Europe. Today issues of biomass production and C sequestration as well as the question of whether these species could increase the adaptive capacity of forests to long-term climate change patterns have fuelled a growing interest in non-native tree species in Europe. In order to determine their fullest potential (and associated management options, but also assess associated risks and challenges) the need for a communication platform allowing for discussion with stakeholder groups from within anf beyond European borders, is argued.
Duration: 3.6.2014 - 13.5.2018

Response of Norway spruce somatic embryos to abiotic stresses
Odpoveď somatických embryí smreka obyčajného na abiotické stresy
Program: Inter-academic agreement
Project leader: Ing. Moravčíková Jana PhD.
Annotation:The aim of the project is to evaluate the response of Norway spruce somatic embryos to abiotic stresses. Somatic embryos will be exposed to different desiccation treatments (high temperature and decreased humidity) and to different doses of UV-B radiation. The alterations of activities of polyamine biosynthetic enzymes and contents of polyamines, abscisic acid (a key endogenous messenger in a plant's response to stresses) and the extent of lipid peroxidation will be evaluated in somatic embryos cultured in stress conditions. The histochemical tests for the presence of polyphenolic substances after the exposure of embryos to UV-B radiationwill complete the biochemical analyses.The collaboration with the Slovak group will complement the research with studies of the family of chitinases at molecular level that will enable to study contemporarily the isoforms important for embryo development as well as dynamics of ongoing stress. The use of somatic embryos will facilitate detailed studies of early physiological responses to various kinds of environmental stresses.
Duration: 1.1.2015 - 31.12.2017

The optimization of the conditions for in vitro reproduction and cultivation of non-traditional fruit lianes Actinidia arguta and Schisandra chinensis cultivars (Ukrainian selection)
Optimalizácia podmienok pre in vitro množenie a kultiváciu netradičných odrôd ovocných lián Actinidia arguta a Schisandra chinensis (ukrajinský výber)
Program: Bilateral - other
Project leader: RNDr. Libiaková Gabriela CSc.
Annotation:This project will initiate cooperation between Institutions in the area of plant micropropagation and will promote level of research. The realization of the project will enable to successfully propagate and cultivate the valuable species for human health (Actinidia arguta and Schizandra chinensis), which will have commercial effect and social and ecological value.
Duration: 6.4.2017 - 6.4.2019

Remarkable elements of lichenbiota of important biotopes of Slovakia and the Czech Republic (SAV-AV ČR 16-07
Pozoruhodné prvky lichenobioty významných biotopov Slovenska a Českej republiky projektu SAV-AV ČR 16-07
Program: Inter-academic agreement
Project leader: Mgr. Košuthová Alica PhD.
Duration: 1.1.2016 - 31.12.2018

Contribution of IB SAS to the international project Millenium Seed Bank
Príspevok BÚ SAV k medzinárodnému projektu Millenium Seed Bank
Program: Other
Project leader: Ing. Kučera Jaromír PhD.
Annotation:The MSB project is the largest ex situ plant conservation programme in the world. Project focus is on global plant life faced with the threat of extinction and plants of most use for the future. The seeds we save are conserved in seed banks as an insurance against the risk of extinction in their native habitat. Working with network of partners across 80 countries, were successfully banked over 13% of the world's wild plant species. The main goal of the project is to save 25% of those species with bankable seeds by 2020 (75,000 species). IB SAS coordinate seeds colection actvities in Slovakia. Target taxa for seed collecting are endemics, endangered and invasive plants. Seeds are stored at MSB Kew and at Gene Bank in Piešťany. Since 2007 were collected and stored from Slovakia 752 taxa. In future we plan to cover with cooperation of several Institutions from relevant countries whole Carpathians area as well as Balkan Peninsula.
Duration: 1.1.2007 - 31.12.2020

Somatic embryogenesis in selected conifer species: initiation of embryogenic tissues, their maintenance, maturation of somatic embryos and plantlet regeneration
Somatická embryogenéza vybraných druhov ihličnatých drevín: indukcia embryogénnych pletív, ich udržiavanie, maturácia somatických embryí a regenerácia rastlín
Program: Inter-academic agreement
Project leader: RNDr. Salaj Terézia DrSc.
Annotation:Somatic embryogenesis (SE) is an in vitro process of embryo differentiation from somatic cells of a plant body. In conifers the process involves several steps as: initiation of embryogenic tissues, their maintenance, somatic embryo maturation and plantlet (somatic seedlings) regeneration. Each step requires specific conditions and is affected by several factors. The submitted proposal intends to study the individual steps of SE, mostly the effect of primary explant developmental stage, plant growth regulators influence as well as osmotic potential of the culture medium. Species as Abies alba, Pinus sylvestris and Pinus nigra will be included as experimental material.
Duration: 1.1.2016 - 31.12.2018

Stress-inducible expression of arabinogalactans and defence-related proteins in relation to androgenesis initiation
Stresom-indukovaná expresia arabinogalaktanov a obranné proteíny v súvislosti s iniciáciou androgenézy
Program: Inter-academic agreement
Project leader: Ing. Moravčíková Jana PhD.
Annotation:Understanding of mechanisms leading to plant embryogenesis under inducible stress conditions (e.g. temperature and/or osmotic stress) at the molecular level is crucial in crop improvement. To overcome resistance of genotypes to microspore embryogenesis (ME) under in vitro conditions, selectable markers for responsiveness/recalcitrance are essential to be identified. Pathogenesis-related proteins (PRs, PR3; chitinases) involved in the plant defence (a)biotic responses with arabinogalactans (AGPs), probably functioned in cell division, differentiation and development, seem to be good candidates. The aim of this project is to characterize the expression of PR3 and AGPs in relation to ME induction effectiveness in rye (Secale cereale L.).
Duration: 1.1.2016 - 31.12.2017

Developing new genetic tools for bioassessment of aquatic ecosystems in Europe
Vývoj nových genetických nástrojov na hodnotenie stavu bioty vodných ekosystémov Európy
Program: COST
Project leader: RNDr. Čiampor Fedor PhD.
Duration: 20.10.2016 - 19.10.2020

The total number of projects: 11