Information Page of SAS Organisation

Institute of Neurobiology

International projects

Neuroprotective potential of cryopreserved placental mesenchymal stem cells (MSCs), extract, cord blood serum in the spinal cord injury (SCI).
Neuroprotektívne potenciál zamrazených mezenchýmových kmeňových buniek (MSCs) placenty, extraktov, séra pupočníkovej krvi pri poranení miechy (SCI).
Program: Medziakademická dohoda (MAD)
Project leader: RNDr. Slovinská Lucia PhD.
Annotation:Determine the neuroprotective potential of cryopreserved placental MSC, placental extract and cord blood serum with spinal cord injuries. Determine the neuroprotective potential of cryopreserved placental MSC, placental extract and cord blood serum with spinal cord injuries.
Duration: 1.1.2017 - 31.12.2019

Targeting molecular pathways of glycolipotoxicity by a novel carboxymethylated mercaptotriazinoindole inhibitor of aldo-keto reductase AKR1B1 in diabetes, inflammation and age-related neurodegeneration.
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Program: Bilaterálne - iné
Project leader: RNDr. Račeková Enikö CSc.
Duration: 15.4.2016 - 15.4.2019

Endurance Training and/or administration of neurotrophic factor as a novel therapeutic approach for treatment of Spinal Cord Injury
Vytrvalostný tréning a/alebo aplikácia rastových faktorov ako nové terapeutické prístupy v liečbe miechy po jej poranení
Program: Bilaterálne - iné
Project leader: RNDr. Lukáčová Nadežda DrSc.
Annotation:Neurotrophic factors (NF) are known to mediate the effect of physical activity on neurogenesis and motor function. Our working hypothesis is that endurance training and/or the administration of NF into selected spinal cord regions after spinal cord injury will enhance the level of NF and improve functional recovery after spinal trauma. While the levels of NF and their receptors are spontaneously down regulated at the site of injury, we anticipate that endurance training enhance the expression of NF and their receptors in motoneurons of spinal cord.
Duration: 1.1.2016 - 31.12.2018

Developing of innovative method of spinal cord injury effective treatment.
Vývoj inovatívnej metódy na efektívnu liečbu poškodenia miechy.
Program: Medziakademická dohoda (MAD)
Project leader: RNDr. Gálik Ján CSc.
Duration: 1.1.2017 - 31.12.2019


National projects

Analysis of post-traumatic inflammatory and regenerative processes along the rostro-caudal axis of the spinal cord after administration of mesenchymal stem cells: an immunohistochemical and proteomic study
Analýza post-traumatických zápalových a regeneračných procesov pozdĺž rostro-kaudálnej osi miechy po podaní mezenchýmových kmeňových buniek: imunohistochemická a neuroproteomická štúdia.
Program: VEGA
Project leader: RNDr. Blaško Juraj PhD.
Annotation:Spinal cord injury (PM) leads to sensory-motor disfunction with lifetime consequences of disability. Due to the complex pathological processes, most treatments are ineffective. The main objective is to modulate several post - traumatic factors in the segments above and below the spinal cord injury by means of cellular therapy (mesenchymal stem cells - MSCs). We will explain the mechanisms of action of the MSC and their products in order to : i ) reduce pro-inflammatory cytokines release from microglia / macrophages in the PM and ii) stimulate nerve fibers overgrowth within in vitro and in vivo systems. By means of mass spectrometry, immunohistochemistry and image analysis techniques, we will obtain a global map of chemokines, cytokines and trophic factors before and after application of cellular therapy. Our research in preclinical in vitro and in vivo models will contribute to the creation of new therapeutic approaches in regenerative medicine with the potential for transfer to clinics.
Duration: 1.1.2015 - 31.12.2017

STEMCELL - Investigation of potential and role of the central canal lining in the reaction to the spinal cord injury
Analýza potenciálu a úlohy výstelky centrálneho kanála pri regenerácii miechy po poranení
Program: APVV
Project leader: RNDr. Račeková Enikö CSc.
Duration: 1.7.2016 - 30.6.2020

Experimentálna t - The application of combined therapy to suppress secondary damage after spinal cord trauma
Aplikácia kombinovanej terapie na potlačenie sekundárneho poškodenia miechy po traume
Program: APVV
Project leader: RNDr. Lukáčová Nadežda DrSc.
Annotation:Despite progress made in the understanding of the pathogenesis of traumatic spinal cord injury in humans, and in improvements of its early diagnostics, we still do not have effective treatment. Mechanisms of secondary injury, as a consequence of primary injury, represent extensive windows for therapeutic manipulations by appropriate exogenous and endogenous interventions. We intend to promote axon regeneration and functional recovery after SCI by blocking the signaling pathways for axonal growth inhibitors in combination with therapeutic effect of the biodegradable material, gradually releasing neurotrophic factors. Because the growth of axons into a spinal cord lesion can be disorganized, we suppose, that long term application of weak electrical field (ES) over injurz site can support their arrangement in linear alignment present in the intact spinal cord. In addition, our objective is to guide the axonal alignment, by AAV9 - GDNF vector, applied subpialy in the vicinity of traumatic lesion. We suppose, that the use of adeno-associated viral (AAV) vector allow long-term and stable transgene expression of GDNF and in combination with ES can be promising intervention for neurogenesis and remyelination, and functional recovery.
Duration: 1.7.2016 - 30.6.2020

Blood as a medium providing the tolerance in a brain after global and focal ischemic attack.
Krv ako médium sprostredkujúce toleranciu v mozgu po globálnom a fokálnom ischemickom zásahu
Program: VEGA
Project leader: RNDr. Bonová Petra PhD.
Annotation:Presented project is focused on the study of mechanisms inducting ischemic tolerance in the models of global and focal brain ischemia in rat. The main goal is determination of mechanism providing information transfer that participates on the creation of brain tolerance to ischemic condition. Based on the knowledge that ischemic tolerance can be induced in any tissue or organ using the broad spectrum of indirect interventions, we will focus on the study of circulating blood and its compartments as the only one medium that fits to the access flexibility. In addition to the source identification and identity of factor inducing ischemic tolerance, our intention is to describe how is this information transmitted to the nerve tissue (overcoming natural barriers), and how the machinery itself triggers induction of the tolerance in different neuronal populations affected by ischemia. For fulfillment the objectives we want to use complex strategy involving several biochemical, proteomic and histological approach.
Duration: 1.1.2015 - 31.12.2017

Localization and role of endothelial nitric oxide synthase in the neurogenic region of the rat during postnatal period
Lokalizácia a úloha endotelovej syntetázy oxidu dusnatého v neurogénnej oblasti potkana v postnatálnom období
Program: VEGA
Project leader: RNDr. Martončíková Marcela PhD.
Duration: 1.1.2017 - 31.12.2019

Neuroprotective mechanisms of the AT2 receptor stimulation after traumatic spinal cord injury.
Neuroprotektívne mechanizmy zahrnuté v stimulácii AT2 receptora po traumatickom poškodení miechy.
Program: VEGA
Project leader: RNDr. Pavel Jaroslav PhD.
Annotation:There is a prolonged social as well as medical demand on intensive research, because no effective and trustworthy clinical treatment for patients with the spinal cord injury (SCI). Since most of the physiological and pathological effects of the Angiotensin II are mediated through the AT1 receptor stimulation, the AT2 receptor study was until recently at the periphery of scientific interest. At present, the AT2 receptor represents a potentially important area of investigation with possible therapeutic application since it was shown its wide neuroprotective potential. Our main objective is to contribute to the elucidation of the neuroprotective mechanisms in which the AT2 receptor plays an important role.
Duration: 1.1.2016 - 31.12.2018

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Nová kombinovaná terapia na báze alginátových biomateriálov a trofických faktorov pre obnovu poranenej miechy.
Program: APVV
Project leader: MVDr. Čížková Dáša DrSc.
Duration: 1.1.2016 - 31.12.2017

Ischemic injured rat brain tissue response to application of postconditioning: the study of ischemic tolerance acquisition with MALDI bottom up proteomic approach
Odpoveď ischemicky poškodeného mozgového tkaniva na aplikáciu postkondicionéra: štúdium mechanizmov získania ischemickej tolerancie MALDI bottom up proteomickým prístupom
Program: VEGA
Project leader: MVDr. Némethová Miroslava PhD.
Annotation:Study of formation and possibilities of ischemic tolerance protective mechanisms participating in the brain cells defense after ischemic injury is basic idea of the project. Project is built on the finding that an end-effector for ischemic tolerance acquisition depends on second stress incidence. It means that sublethal stress if applicated within 2 days after the lethal one can have protective effect against delayed neuronal death. Specifically, we will focus on protein profiling in the most ischemic-reperfusion injury sensitive brain areas (e.g. CA1), as well as in the relative resistant regions (dentate gyrus) by the mass spectrometry proteomic analysis. We suppose that proteomic pattern allows us to understand mechanisms, which are involved in targeted protection and survival of neurons. Characterization of changes in protein profiles could contribute to identification of molecular processes which are participating in stress response regulation and are involved in ischemic reperfusion pathophysiology.
Duration: 1.1.2015 - 31.12.2017

REGENER - Axonal regeneration in biosynthetic nerve guide conduits.
Regenerácia nervových vlákien v biosyntetických vodičoch.
Program: APVV
Project leader: MVDr. Vanický Ivo CSc.
Annotation:In adult mammals, nerve fibers are capable of regeneration. In peripheral nerve after injury, axons from the proximal stump regrow across the site of injury into the distal stump and create new connections with their target organs. Current surgical techniques allow for a direct reconstruction of the severed nerve (end-to-end) only if the missing segment is not too large. After devastating injuries, autologous nerve grafts can be used. Synthetic nerve guides for nerve regeneration can be used as a substitute for autologous grafts. Regrowth of regenerating fibers across the synthetic nerve guides is limited to relatively short distances. This restriction is surprising, because in the distal stumps, regenerating axons are able to grow over long distances. Advances in the synthesis of biocompatible polymers provide new biomaterials with optimized properties. In the present project, we plan to use biosynthetic guides made from new elastomeric materials. The properties of the nerve guide will be modified and tested in vitro, with the goal to stimulate regeneration over long distances. The effectiveness of these modifications on regeneration process will be tested in vivo. 15 Žiadateľská organizácia Neurobiologický ústav SAV Co-ordinating organization Slovak Academy of Sciences, Institute of Neurobiology
Duration: 1.7.2015 - 30.6.2019

Investigation of postnatal neurogenesis in relation to neurodegeneration
Skúmanie postnatálnej neurogenézy vo vzťahu k neurodegeneráciam
Program: VEGA
Project leader: RNDr. Račeková Enikö CSc.
Annotation:The generation and death of newborn cells have critical roles in brain development and maintenance in the adult brain. Specific alterations in these processes are seen in neurodegenerative diseases. The pathogenesis of these diseases is not yet known. It is assumed that the phenomenon of postnatal neurogenesis, respectively its failure may be associated with the development of neurodegenerative diseases. Our previous findings have demonstrated that adverse environmental factors can induce changes in the neurogenesis in the rat olfactory system. The aim of the proposed project is to verify the hypothesis that there is a link between neurogenesis and neurodegeneration. We will investigate if specific alterations in the olfactory system neurogenesis parallel the early symptoms of some neurodegenerative disease, such as depression, anxiety or olfactory dysfunction. The obtained results could contribute to understanding of the role of postnatal neurogenesis in the pathogenesis of neurodegenerative diseases.
Duration: 1.1.2015 - 31.12.2017

The therapeutic effects of stem cells conditioned medium on the repair of the spinal cord damaged tissue: a comparative ex vivo study.
Terapeutické účinky kondiciovaného média kmeňových buniek na reparáciu poškodeného tkaniva miechy: porovnávacia ex vivo štúdia.
Program: VEGA
Project leader: RNDr. Slovinská Lucia PhD.
Annotation:Spinal cord injury is a neurodegenerative disease of the CNS, which leads to damage and death of the spinal cord cell population, namely neurons, oligodendrocytes, astrocytes. Cell therapy is a key area of regenerative medicine to find suitable sources of transplanted cells or their products, allowing restoration of damaged tissue. The aim of the present project is to establish an ex vivo model of traumatic injured CNS tissue in the form of organotypic spinal cord slices. Using this model we will investigate the effects of the conditioned medium of the stem cells (mesenchymal stem cells from bone marrow and adipose tissue, neural progenitors) on the repair of damaged rat spinal cord tissue. We compare the effectiveness of different conditioned media on the base of survival and proliferative potential of glial cell population, neurons and nerve fibers overgrowth. We suppose, that the ex vivo model of mechanical damage will be a good method for the study of potential therapeutic strategies in CNS damage.
Duration: 1.1.2016 - 31.12.2018

The effect of GDNF vector and block of inhibition molecules on interneuronal connections and axonal outgrowth after cervical and thoracic spinal cord injury
Účinok GDNF vektora a blokovania inhibičných molekúl na interneuronálne prepojenia a prerastanie axónov po cervikálnom a torakálnom poškodení miechy
Program: VEGA
Project leader: RNDr. Lukáčová Nadežda DrSc.
Annotation:The aim of proposed project is 1) to examine molecular changes in the respiratory nuclei and interneurons, and reinervation of bulsbospinal pathway after cervical compression and application of AAV9-GDNF vector that allow long-term and stable transgene expression of GDNF in the vicinity of the SCI lesion, 2) to promote axonal regeneration and to improve functional recovery after thoracic compression by blocking the signaling pathways for axonal growth inhibitors. We will compare two ways (intraparenchymal and subpial) of application, and in combination with post-traumatic rehabilitation training we will determine, which interneuronal connections (commissural, propriospinal, reticulospinal) promote functional outcome.
Duration: 1.1.2017 - 31.12.2019

Influence of electromagnetic radiation on some organs of sexually immature rats
Vplyv elektromagnetickej radiácie na štruktúru niektorých orgánov pohlavne nedospelých potkanov
Program: VEGA
Project leader: RNDr. Račeková Enikö CSc.
Duration: 1.1.2015 - 31.12.2017

Projects total: 17