Electronic Library of Scientific Literature - © Academic Electronic Press


Volume 35 / No. 3 / 2001



Francesca Fernandez, Marlene Durand, Valerie Coomans, Pierre Mormede, Francis Chaouloff

NeuroGenetics & Stress, INSERM U471-INRA, 33077 Bordeaux Cιdex, France
E-mail: francis.chaouloff@bordeaux.inserm.fr

Objective. There is evidence for a regulatory impact of corticoids on hippocampal serotonergic systems, including serotonin (5-HT) synthesis and 5-HT1A receptor expression and/or activity. On the other hand, contradictory data have emerged as to the regulation of the 5-HT transporter by corticoids. Using male Spontaneously Hypertensive Rats (SHRs) and Wistar-Kyoto (WKY) rats, we have analysed whether subchronic corticosterone alters in a strain-dependent manner the reuptake activity of the hippocampal 5-HT transporter.
Methods. Two separate experiments were performed. In the first experiments, we assessed the impact of corticosterone ingestion (400 ΅g/ml in drinking water for 7 days) on hippocampal reuptake of increasing concentrations of [3H]5-HT (6.25-100 nM). In the second series of experiments, we measured whether such a corticosterone regimen affected the potency of the antidepressant citalopram to block the reuptake of 10 nM of [3H]5-HT.
Results. Corticosterone administration, which markedly reduced body weight gains and adrenal weights in both strains, increased Km and Vmax values in SHRs but decreased these values in WKY rats, compared to vehicle (2.4 % ethanol) administration. In addition, it was observed that neither the basal reuptake of 10 nM [3H]5-HT nor the potency of citalopram to block selectively such a reuptake (IC50 = 2.88-3.63 nM) differred between vehicle- and corticosterone-treated animals.
Conclusion. Under our experimental conditions, both the reuptake of a physiological concentration of 5-HT and the potency of an antidepressant to inhibit such a reuptake proved insensitive to repeated corticosterone administration.
Key words: Corticosterone – [3H]Serotonin reuptake – Hippocampus – Citalopram – Wistar Kyoto Rats – Spontaneously Hypertensive Rats

ENDOCRINE REGULATIONS, Vol. 35, 119–126, 2001

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Angelina I. Russinova, Christina A. Valkova, Roumiana T. Denkova

Institute of Experimental Morphology and Anthropology, Department of Cell Differentiation, Bulgarian Acadamy of Sciences and
Institute of Molecular Biology, Department of Cell Biology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

Objective. In the present study we employed a monoclonal antibody (Mab 3D8) obtained against a rat ovarian antigen and indentified a 76 kDa protein in porcine ovarian follicles.
Methods. The localization of this antigen was studied by light and electron-microscopic immunocytochemistry and further characterized by polyacrylamide gel elctrophoresis and immunoblotting on nitrocellulose membranes.
Results. We found that the antigen recognized by Mab 3D8 is localized in granulosa cells (GCs) and oocytes. The expression of the 76 kDa protein apparently depends on the developmental stage. A particularly strong reaction was observed in cumulus cells and oocytes in early and late antral follicles. In granulosa cells the reaction product was localized in rough endoplasmic reticulum (RER), cis and trans faces of the Golgi stack, the outer nuclear envelope and in numerous transport vesicles budding from the endoplasmic reticulum. In the oocyte the reaction product was localized in structures related to specific endocytosis – small pits at the cell surface, two subsets of endosomes, endocytic carrier vesicles and the prelysosomal compartment (PLC).
Conclusions. The results obtained suggest that porcine oocytes possess the cellular structures which allow them to bind and internalize this protein, which is most probably produced by granulosa cells. During oocyte development the intracellular site of accumulation of the 76 kDa protein varies, which implies that it is under developmental control.
Key words: Monoclonal antibody – Immunocytochemistry – Endocytosis- Porcine ovarian follicle

ENDOCRINE REGULATIONS, Vol. 35, 127–137, 2001

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Marek Pawlikowski, Anna Gruszka, Slavomir Mucha, Gabriela Melen-Mucha

Department of Experimental Endocrinology and Hormone Diagnostics and Department of Clinical Endocrinology, Institute of Endocrinology, Medical University of Lodz, 91425 Lodz, Poland
E-mail: m.pawlikowski@mail.e.pl

Objective. The effects of angiotensins II (AngII) and IV (Ang IV,3-8 fragment of angiotensin II) on the adrenocortical cell proliferation have been investigated in the rat.
Methods. The male adult Wistar rats were injected subcutaneously with saline, captopril or captopril together with either Ang II or Ang IV. A part of animals received additionally losartan – an antagonist of AT1 subtype of angiotensin receptors. Bromodeoxyuridine (BrDU) incorporation into cell nuclei was used as the index of cell proliferation.
Results. It was found that both Ang II and Ang IV increased the BrDU labeling in the adrenal cortex of captopril-pretreated rats. This effect involved mainly the zona glomerulosa cells.The proliferogenic effect of Ang II was blocked by AT1 receptor antagonist losartan. In contrast, losartan did not block the effect of Ang IV.
Conclusion. Both Ang II and Ang IV stimulate the adrenocortical cell proliferation in the rat, but they act via different receptors – AT1 in the case of Ang II and non-AT1 (probably AT4) in the case of Ang IV.
Key words: Adrenal cortex – Cell proliferation – Angiotensin II – Angiotensin IV

ENDOCRINE REGULATIONS, Vol. 35, 139–142, 2001

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Josef Thalhamer, Wolfgang Leitner, Peter Hammerl, Julius Brtko

Immunology Group, Institute of Chemistry and Biochemistry, University of Salzburg, Austria,
Surgery Branch, National Cancer Institute, NIH, Bethesda MD 20892, USA
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic
Email: Josef.Thalhamer@sbg.ac.at

Genetic immunization or DNA vaccination represents a rapidly developing technology with new perspectives for the prevention and therapy of infectious diseases and it offers new approaches for the treatment of autoimmunity, tumors and even allergy. DNA vaccines are comprised of plasmid DNA which encodes antigen molecules directly in the transfected cells of a target organism. In contrast to protein-induced immune responses, DNA vaccines stimulate both humoral and cell-mediated immune reactions.
In the present review we present a palette of unique features of genetic immunization like the effect of CpG motifs, the influence of mode and site of gene delivery and the modulation of immune responses by co-delivery of cytokines, colony stimulating factors, adhesion molecules and other stimulatory molecules. In addition, modulation of the immune response via translation, processing and presentation will be discussed, which in sum demonstrate the elegant possibilities of genetic immunization to induce tailor-made immune responses.

ENDOCRINE REGULATIONS, Vol. 35, 143–166, 2001

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Magdalena Matejkova-Behanova

Institute of Endocrinology, 116 94 Prague, Czech Republic
E-mail: mbehanova@endo.cz

Latent autoimmune diabetes in adults (LADA) is characterised by clinical presentation as type 2 diabetes after 25 years of age, initial control achieved with diet or oral hypoglycaemic agents during at least 6 months, presence of autoantibodies (first of all GADA) and some immunogenetic features of diabetes mellitus type 1. In patients with an autoimmune endocrine disease, which could be also autoimmune diabetes, there is a high risk of development of another autoimmune endocrine disorder. The coexistence of two or more autoimmune endocrine diseases is pathognomonic for autoimmune polyglandular syndrome. Autoimmune thyroiditis and type 1 diabetes mellitus are the most common combination of autoimmune endocrine diseases reported. Most studies reported the prevalence of autoimmune thyroiditis in ”typical” type 1 adult diabetic subjects about 20 – 40%. Little is known about the prevalence of autoimmune thyroiditis in subjects with LADA. Only a few studies confirmed a high prevalence of thyroid autoantibodies in type 2 diabetic subjects with GADA compared to type 2 diabetic subjects without GADA and compared to non-diabetic population too.
Key words: Diabetes mellitus type 1 – LADA – Autoimmune thyroiditis – Autoimmune polyglandular syndrome

ENDOCRINE REGULATIONS, Vol. 35, 167–172, 2001

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