In vitro and in vivo studies of antitumor effects of the recombinant immunotoxin MSH-PE38KDEL on melanoma

Rok, strany: 2014, 392 - 400

MSH-PE38KDEL is a chimeric molecule composed of MSH, and fused to a truncated mutant form of Pseudomonas exotoxin (PE38KDEL). Our study aims to evaluate the specific cytotoxicity of recombinant immunotoxin MSH-PE38KDEL on melanoma cells A875 and B16 in vitro, as well as its inhibition of metastatic melanoma in vivo. MSH-PE38KDEL was expressed in Escherichia coli, and greater than 90% purity was obtained. The purified MSH-PE38KDEL was found to be selectively cytotoxic to MSH receptor-positive melanoma cells in vitro. The specific cytotoxicity of recombinant MSH-PE38KDEL to A875 and B16 was over 85% by cell viability assay; however, MSH-PE38KDEL had no cytotoxicity to the human 2BS cells. The anti­tumor activity of MSH-PE38KDEL was evaluated in mice with induced melanoma through intra-tumor or intravenous administration. The results showed that 90% melanoma growths were inhibited, and 40% of the tumors were disappeared completely. Histopathology results showed MSH-PE38KDEL can effectively inhibit intrahepatic metastasis. In conclusion, MSH-PE38KDEL had cytotoxic effects on MSH receptor-positive melanoma cells, and causes significant tumor growth inhibition. These results support a possible new approach for the treatment of melanoma. Keywords: recombinant immunotoxins, MSH-PE38KDEL, melanoma, antitumor

ISO 690:

Hui, Q., Ma, J., Song, J., Liu, Z., Ren, H., Jiang, W., Wang, Y., Xu, Y., Guo, D., Zhang, X., Lu, S. 2014. In vitro and in vivo studies of antitumor effects of the recombinant immunotoxin MSH-PE38KDEL on melanoma. In NEOPLASMA, vol. 61, no.4, pp. 392-400. 0028-2685.

APA:

Hui, Q., Ma, J., Song, J., Liu, Z., Ren, H., Jiang, W., Wang, Y., Xu, Y., Guo, D., Zhang, X., Lu, S. (2014). In vitro and in vivo studies of antitumor effects of the recombinant immunotoxin MSH-PE38KDEL on melanoma. NEOPLASMA, 61(4), 392-400. 0028-2685.