Clonal evolution in chronic lymphocytic leukemia studied by interphase fluorescence in-situ hybridization

Rok, strany: 2009, 455 - 458

The results of repeated interphase fluorescence in-situ hybridization (I-FISH, FISH) examination of 97 CLL patients and correlation of these findings with IgVH hypermutation status, ZAP-70 and CD38 expression are presented. The appearance of new, FISH-detectable, genomic aberrations during disease course, described as clonal evolution (CE), was observed in 26% of patients. The most frequent newly acquired cytogenetic abnormality was 13q deletion in 64% (16/25). In contrast to earlier studies, there was no correlation found between CE and either one of single negative prognostic factors (unmutated IgVH; CD38 positivity; ZAP-70 positivity). However, the combination of all three negative factors correlated with CE highly significantly (p=0.005) and moreover, also with a shift from lower to higher FISH risk category (p=0.010). As the prognostic data were known in all patients, this study represents the complete insight on the association of CE and other risk parameters in CLL. Keywords: CLL; FISH; clonal evolution; prognostic markers; high-risk deletions

ISO 690:

Berkova, A., Zemanova, Z., Trneny, M., Schwarz, J., Karban, J., Cmunt, E., Pavlistova, L., Brezinova, J., Michalova, K. 2009. Clonal evolution in chronic lymphocytic leukemia studied by interphase fluorescence in-situ hybridization. In NEOPLASMA, vol. 56, no.5, pp. 455-458. 0028-2685.

APA:

Berkova, A., Zemanova, Z., Trneny, M., Schwarz, J., Karban, J., Cmunt, E., Pavlistova, L., Brezinova, J., Michalova, K. (2009). Clonal evolution in chronic lymphocytic leukemia studied by interphase fluorescence in-situ hybridization. NEOPLASMA, 56(5), 455-458. 0028-2685.