In: NEOPLASMA, vol. 54, no. 1
M. Cichorek - K. Kozlowska - E. Bryl
Detaily:
Rok, strany: 2007, 29 - 36
O článku:
In this work we tried to estimate the role of mitochondria in the ability of cells of two: melanotic and amelanotic
transplantable melanoma lines to undergo spontaneous and camptothecin-induced apoptosis.
We measured mitochondrial transmembrane potential (ΔΨ) changes during culture without (spontaneous apoptosis) and
with camptothecin (induced apoptosis) by using JC-1 staining and flow cytometry analysis. Cytochrome c release and
PARP cleavage as the biological effects of ΔΨm changes belonging to the phenomena observed during apoptosis were
estimated by Western blotting.
The results of our investigations showed in both transplantable melanoma cells the features indicating apoptosis: ΔΨ
changes, cytochrome c release and PARP cleavage, but the degree of observed changes depended on the phenotype of
melanoma cells examined. After camptothecin treatment the changes were more pronounced in the amelanotic melanoma cells- the more aggressive line.
Key words: mitochondrial transmembrane potential, transplantable melanomas, apoptosis, camptothecin
Ako citovať:
ISO 690:
Cichorek, M., Kozlowska, K., Bryl, E. 2007. Mitochondrial transmembrane potential in spontaneous and camptothecininduced
apoptosis of melanotic and amelanotic melanoma cells.. In NEOPLASMA, vol. 54, no.1, pp. 29-36. 0028-2685.
APA:
Cichorek, M., Kozlowska, K., Bryl, E. (2007). Mitochondrial transmembrane potential in spontaneous and camptothecininduced
apoptosis of melanotic and amelanotic melanoma cells.. NEOPLASMA, 54(1), 29-36. 0028-2685.