In: NEOPLASMA, vol. 53, no. 6
G. Che - J. Chen - L. Liu - Y. Wang - L. Li - Y. Qin - Q. Zhou
Detaily:
Rok, strany: 2006, 530 - 537
O článku:
Nm23 is a metastasis suppressor gene. In this report, we transfected nm23-H1 cDNA into L9981, a human large cell lung
cancer cell line with nm23 negative expression, and made a stable transfectant. L9981-nm23-H1 cells exhibited lower cells
proliferation rate, more G0/G1 phase growth and an increase in apoptosis with a dramatic decreased in the tumor cells ability
to metastasize. L9981-nm23-H1 cells also demonstrated a significantly reduced lymph node and pulmonary metastatic capacity
in vivo when injected into the nude mice. Furthermore, we used DNAmicroarray analysis to explore the change in expression
of the metastasis-related genes in L9981-nm23-H1 cells. We found that the expression of beta-Catenin, E-Cadherin
and TIMP-1 were significantly increased while expression MMP-2, CD44v6, and VEGF was dramatically decreased in
L9981-nm23-H1, as confirmed by RT-PCR and western blot. These results demonstrated that nm23-H1 can suppress the
mobility and metastatic capacity of cancer cells and the molecular mechanism by which nm23-H1 suppresses tumor metastasis
may be via increasing the expression of metastasis-related genes such as beta-Catenin, E-Cadherin and TIMP-1 and decreasing
the expression of MMP-2, CD44V6 and VEGF.
Ako citovať:
ISO 690:
Che, G., Chen, J., Liu, L., Wang, Y., Li, L., Qin, Y., Zhou, Q. 2006. Transfection of nm23-H1 increased expression of beta-Catenin, E-Cadherin and TIMP-1 and decreased the expression of MMP-2, CD44v6 and VEGF and inhibited the metastatic potential of human non-small cell lung cancer cell line L9981. In NEOPLASMA, vol. 53, no.6, pp. 530-537. 0028-2685.
APA:
Che, G., Chen, J., Liu, L., Wang, Y., Li, L., Qin, Y., Zhou, Q. (2006). Transfection of nm23-H1 increased expression of beta-Catenin, E-Cadherin and TIMP-1 and decreased the expression of MMP-2, CD44v6 and VEGF and inhibited the metastatic potential of human non-small cell lung cancer cell line L9981. NEOPLASMA, 53(6), 530-537. 0028-2685.