In: NEOPLASMA, vol. 53, no. 6
J. Jakubikova - Y. Bao - J. Bodo - J. Sedlak
Detaily:
Rok, strany: 2006, 463 - 470
O článku:
The aim of presented study was to further investigate the concentration-dependent changes induced by isothiocyanate
iberin (IBN) in human colon carcinoma Caco-2 cells. The concentrations of IBN below IC50 value (18 microM, 72 h) triggered
the augmentation of mRNA levels for phase II detoxification GSTA1 and UGT1A1 enzymes and antioxidant thioredoxin
reductase 1 gene in cells treated for 24 h. In addition a significant increase of acetylated H4 histone was detected. The
mRNA induction peaked at IC50 value and returned to level of control cells at 40 microM concentration of IBN. The cell cycle
changes, gamma-H2AX stainability and the increase of phospho-H3 mitotic marker were induced at concentrations above IC50
value. Appearance of Annexin V positive apoptotic cells and sub-G1 fragmented DNA as well as decrease of mitochondrial
transmembrane potential confirmed cytotoxic effect of IBN observed in MTT assay. The predominance of necrotic cells and
profound positivity of gamma-H2AX took place at the highest concentration of IBN. Thus, IBN represents the effective member
of natural chemopreventive isothiocyanate family with which apoptotic potential can by employed to eliminate tumor cells.
Ako citovať:
ISO 690:
Jakubikova, J., Bao, Y., Bodo, J., Sedlak, J. 2006. Isothiocyanate iberin modulates phase II enzymes, posttranslational modification of histones and inhibits growth of Caco-2 cells by inducing apoptosis. In NEOPLASMA, vol. 53, no.6, pp. 463-470. 0028-2685.
APA:
Jakubikova, J., Bao, Y., Bodo, J., Sedlak, J. (2006). Isothiocyanate iberin modulates phase II enzymes, posttranslational modification of histones and inhibits growth of Caco-2 cells by inducing apoptosis. NEOPLASMA, 53(6), 463-470. 0028-2685.