In: NEOPLASMA, vol. 53, no. 5
L. Kozlowski - T. Filipowski - M. Rucinska - W. Pepinski - J. Janica - M. Skawronska - J. Poznanski - M.z. Wojtukiewicz
Detaily:
Rok, strany: 2006, 440 - 443
O článku:
Loss of heterozygosity (LOH) has been shown to be an important
prognostic factor in a variety of malignant neoplasm\'s.
Cervical cancer develops as result of multiple genetic
alterations. The aim of this study was to analyze presence of LOH
in
cervical cancer and to identify the correlation between LOH and
survival and relapse-free survival time in patients treated
with radiotherapy. Studies were performed on tumor specimens and
venous blood from 20 patients with cervical cancer
(squamous cell carcinoma G2 and G3) in stage II and III (FIGO)
treated with radiotherapy. DNA was isolated using organic
extraction. Additional microcolumn purification was performed. The
fluorescent multiplex polymerase chain reaction
(PCR) was used to amplify 10 microsatellite loci included in
commercially available human identification kits.
Microsatellite marker BAT 26 was amplified in separate PCR
reactions. 75% cervical cancers manifested LOH. LOH in
BAT 26 analysis (chromosome 2) was present in all these specimens.
60% of the cases showed LOH at one or more of other
examined loci (mostly on 3p, 18q21.3, and 11p15.5). Eight of nine
cervical cancers in clinical stage III showed LOH. All
cases of G3 squamous cell carcinoma of the cervix manifested LOH
on 2p. Patients with LOH have worse prognosis for survival
and relapse-free survival compared to patients without LOH.
Ako citovať:
ISO 690:
Kozlowski, L., Filipowski, T., Rucinska, M., Pepinski, W., Janica, J., Skawronska, M., Poznanski, J., Wojtukiewicz, M. 2006. Loss of heterozygosity on chromosomes 2p, 3p, 18q21.3 and 11p15.5
as a poor prognostic factor in stage II and III (FIGO) cervical
cancer treated by radiotherapy. In NEOPLASMA, vol. 53, no.5, pp. 440-443. 0028-2685.
APA:
Kozlowski, L., Filipowski, T., Rucinska, M., Pepinski, W., Janica, J., Skawronska, M., Poznanski, J., Wojtukiewicz, M. (2006). Loss of heterozygosity on chromosomes 2p, 3p, 18q21.3 and 11p15.5
as a poor prognostic factor in stage II and III (FIGO) cervical
cancer treated by radiotherapy. NEOPLASMA, 53(5), 440-443. 0028-2685.