Effect of RRS1 gene knockdown on BT549 cell line proliferation and apoptosis in breast cancer

In: NEOPLASMA, vol. 66, no. 1

Yn Hua - Jl Song - Zl Ma - L Wu - Z Zhang - L Zhang - N Li - Sb Cong - L Hou

Detaily:

Rok, strany: 2019, 28 - 32

O článku:

The RRS1 regulator of ribosome synthesis has recently been reported a new target gene linked to cancer development. This study therefore investigates RRS1effectsb on BT549 cell proliferation and apoptosis in breast cancer. Western blot (WB) and real - time quantitative PCR (qPCR) were used to detect the relative expression of RRS1 in breast cancer cells BT-549 and the normal HMEC mammary gland epithelial cells. BT-549 cells were cultured and infected with retroviruses and RRS1 expression was detected by qPCR and WB. The MTT assay, Caspase-3/7 and flow cytometry (FCM) then detected growth and apoptosis in the BT549 breast cancer BT cell. WB detected the expression of Bcl-2 and Bax genes related to apoptosis at the protein level, and MTT assay confirmed that RRS1 knockdown significantly decreased cell viability (p<0.05) and induced apoptosis which was rescued by shRNA-RRS1 expression. The amount of caspase-3 increased significantly and apoptosis was obvious. The apoptotic cells amount analyzed by FCM was significantly increased and RRS1 knockdown also decreased the expression of apoptosis related protein bcl-2 and simultaneously increased the expression of Bax (p<0.05). Finally, the RRS1 gene was highly expressed in breast cancer cell line BT549 and its knockdown significantly reduced proliferation and apoptosis in BT549 cell. These results suggest that RRS1 is a novel gene related to breast cancer and has an important role in breast cancer proliferation and apoptosis.

Ako citovať:

ISO 690:

Hua, Y., Song, J., Ma, Z., Wu, L., Zhang, Z., Zhang, L., Li, N., Cong, S., Hou, L. 2019. Effect of RRS1 gene knockdown on BT549 cell line proliferation and apoptosis in breast cancer. In NEOPLASMA, vol. 66, no.1, pp. 28-32. 0028-2685.

APA:

Hua, Y., Song, J., Ma, Z., Wu, L., Zhang, Z., Zhang, L., Li, N., Cong, S., Hou, L. (2019). Effect of RRS1 gene knockdown on BT549 cell line proliferation and apoptosis in breast cancer. NEOPLASMA, 66(1), 28-32. 0028-2685.