MicroRNA 363 mediated positive regulation of c-myc translation affect prostate cancer development and progress

Rok, strany: 2015, 191 - 198

Prostate cancer (CaP) is the sixth most significant cancer killer of men in China. In this study, the potential role of micro-363 (miR-363) in CaP development and progression was investigated. Pri-miR-363 or anti-miR-363 was transfected into the CaP cells line PC-3 cells. Cell proliferation, transformation property, and epithelial-to-mesenchymal transition (EMT) were evaluated by MTT, clonogenic assay, colony formation in soft agar and western blotting, respectively. The expression and involvement of c-myc, a downstream target of miR-363 were also determined. The results showed that endogenous expression of miR-363 was significantly increased in CaP cells compared with normal prostate cells. High expression of miR-363 in PC-3 cells through transfection induces cell proliferation and positively regulates cell transformation property as well as promotes EMT of PC-3 cells. Through knockdown of c-myc, the results also showed that c-myc was involved in the regulation of biological function of PC-3 cells by miR-363. Taken together, this study adds support to the potential role of miR-363 in the diagnosis and treatment of CaP. Keywords: Prostate cancer, transformation property, proliferation, micro-363, epithelial-to-mesenchymal transition

ISO 690:

Chen, Y., Lu, X., Wu, B., Su, Y., Li, J., Wang, H. 2015. MicroRNA 363 mediated positive regulation of c-myc translation affect prostate cancer development and progress. In NEOPLASMA, vol. 62, no.2, pp. 191-198. 0028-2685.

APA:

Chen, Y., Lu, X., Wu, B., Su, Y., Li, J., Wang, H. (2015). MicroRNA 363 mediated positive regulation of c-myc translation affect prostate cancer development and progress. NEOPLASMA, 62(2), 191-198. 0028-2685.