In: NEOPLASMA, vol. 54, no. 6
K. Polakova - E. Bandžuchová - J. Tirpakova - D. Kuba - G. Russ
Detaily:
Rok, strany: 2007, 455 - 462
O článku:
Recent studies demonstrated that HLA-G transcription is in some cells silenced by epigenetic mechanisms as DNA
methylation and histone modification. Accordingly HLA-G gene transcriptions can be activated in such cells by demethylating
agent or by inhibitors of histone deacetylation. In addition to epigenetic alterations HLA-G gene transcription can be activated
by stress. In the present study these aspects of HLA-G expression are re-examined and a new inhibitor of histone deacetylation
(valproic acid) and hypoxia mimetic chemical (CoCl2) are included. The highest activation of HLA-G transcription was
achieved by treatment of choriocarcinoma JAR and lymphoblastoid RAJI cell lines with demethylating agent 5-aza-2´-
deoxycytidine. Treatment of JAR and RAJI cells with histone deacetylase inhibitors (sodium butyrate and valproic acid)
also enhanced HLA-G transcription. Nevertheless this increase in HLA-G expression was low as compared with activation
by 5-aza-2´- deoxycytidine. The hypoxia mimetic agents (desferrioxamine or CoCl2) had no detectable effect on HLA-G
gene transcription in examined cells. Relatively high increase of HLA-G transcription was detected in JAR and RAJI cells
exposed to heat shock treatment. Interestingly heat shock induced high expression of HLA-G6 transcript in JAR cells. Heat
shock treatment had no effect on alternative splicing of constitutively expressed HLA-G mRNA in choriocarcinoma cell line
JEG-3. HLA-G1 protein expression was induced in JAR and RAJI cell lines by 5-aza-2´- deoxycytidine. In agreement with
the differences in the levels of HLA-G transcripts JAR cells express more of HLA-G1 protein than RAJI cells.
Key words: HLA-G; demethylation; histone deacetylation; hypoxia; heat shock
Ako citovať:
ISO 690:
Polakova, K., Bandžuchová, E., Tirpakova, J., Kuba, D., Russ, G. 2007. Modulation of HLA-G expression. In NEOPLASMA, vol. 54, no.6, pp. 455-462. 0028-2685.
APA:
Polakova, K., Bandžuchová, E., Tirpakova, J., Kuba, D., Russ, G. (2007). Modulation of HLA-G expression. NEOPLASMA, 54(6), 455-462. 0028-2685.