In: NEOPLASMA, vol. 53, no. 3
V. Scudla - M. Ordeltova - J. Minarik - L. Dusek - M. Zemanova - J. Bacovsky
Detaily:
Rok, strany: 2006, 213 - 218
O článku:
The aim of this study was a contemporaneous measurement of plasma
cells proliferative and apoptotic activity in patients
examined at the time of multiple myeloma (MM) diagnosis before
initiation of chemotherapy, focussed on the following aspects:
determination of prognostic significance of plasma cell propidium
iodide (PC-PI) and annexin-V FITC (PC-AI) indices;
optimal cut off of PC-PI and PC-AI with regard to overall
survival; calculation of summary kinetic index of plasma cells
(PC-PI/AI ratio) for evaluation of its prognostic importance;
determination of an index (out of PC-PI, PC-AI and PC-PI/AI)
showing the closest relation to prognosis of multiple myeloma.
The analyzed 122 patients fulfilling SWOG multiple myeloma
criteria were treated by conventional chemotherapy.
Plasma cell proliferative activity was measured by means of PC-PI
examined by flow cytometry using a DNA/CD138 double
staining technique. For detection of plasma cells entering
apoptosis (PC-AI), flow-cytometry method with annexin-V FITC
and MoAb CD138 was used.
The PC-PI median in 122 patients was 2.6(0.4—4.8)%. The sequence
prognostic analysis showed that the optimal PC-PI
cut off was 2.9% and displayed a significant relationship with
overall survival (OS) (p=0.031). The group of 94 patients had
PC-AI median of 5.0(1.4—24.5)%. The best statistical significance
of the rate of apoptosis related to overall survival was
found at cut off value of 4.4% (p=0.022). The median of overall
kinetic index of plasma cells (PC-PI/AI) examined in 94
MM patients was 0.5(0.05—2.60) and the overall kinetic index was
found to display a very good relationship to OS at the cut
off value of 0.71 (p=0.032). All the three indices expressing
various aspects of kinetics of plasma cells allow the
stratification
of patients into two prognostically different groups with
statistically significantly different medians of overall survival:
good risk — OS still undeterminable at the time of analysis; bad
risk — M: OS was for PC-PI 17 months, for PC-AI 23 months
and for PC-PI/AI 16 months. The ratio of both indices, i.e. PC-
PI/AI, however did not bring any further contribution to overall
survival/prognosis evaluation, when compared with single PC-PI and
PC-AI.
Results of present study indicates that the evaluation of both
proliferation and apoptotic activities of plasma cells is important
for prognosis thus extending possibilities of initial
stratification ofMMpatients into groups with different prognostic
risk.
Ako citovať:
ISO 690:
Scudla, V., Ordeltova, M., Minarik, J., Dusek, L., Zemanova, M., Bacovsky, J. 2006. Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma. In NEOPLASMA, vol. 53, no.3, pp. 213-218. 0028-2685.
APA:
Scudla, V., Ordeltova, M., Minarik, J., Dusek, L., Zemanova, M., Bacovsky, J. (2006). Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma. NEOPLASMA, 53(3), 213-218. 0028-2685.