In: ENDOCRINE REGULATIONS, vol. 52, no. 4
M. Z. Mohamed - H. M. Hafez - H. H. Mohamed - N. M. Zenhom
Detaily:
Rok, strany: 2018, 192 - 198
Jazyk: eng
Kľúčové slová:
streptozotocin, verapamil, lung, STAT3, TNFα, Nrf2
Typ článku: Research Article
Typ dokumentu: Research Article
O článku:
Objective. We aimed to assess the protective role of verapamil, L-type calcium channel blockers,
against early lung damage in diabetic rats. Lung injury has recently been recognized as a consequent complication of diabetes mellitus. Hyperglycemia induces inflammatory changes in lung tissue early in the disease.
Methods. Twenty four adult male rats were grouped into control, diabetic, diabetic treated with verapamil, and verapamil control. Streptozotocin (STZ) was used to induce diabetes. Oxidative parameters and antioxidative mechanisms were assessed in lung homogenate. Tumor necrosis factor alpha (TNFα) protein was measured as a pro-inflammatory mediator. Signal transducer and activator of transcription 3 (STAT3) gene expression and nuclear erythroid factor 2 (Nrf2) immunoexpression were screened.
Results. The lung showed oxidative damage and inflammatory infiltration in STZ diabetic rats
early at 2 weeks. The parameters significantly improved in lung tissue treated with verapamil.
Histopathology of the lung tissue confirmed the results. Inhibition of STAT3/TNFα pathway was
involved in the protection offered by verapamil. Activation of Nrf2 together with an increasing
antioxidant capacity of diabetic lung significantly ameliorates the injury induced by diabetes.
Conclusions. Verapamil afforded protection in diabetic lung injury. The protection was mediated by the anti-inflammatory and antioxidant effects of verapamil.
Ako citovať:
ISO 690:
Mohamed, M., Hafez, H., Mohamed, H., Zenhom, N. 2018. STAT3 and Nrf2 pathways modulate the protective effect of verapamil on lung injury of diabetic rats. In ENDOCRINE REGULATIONS, vol. 52, no.4, pp. 192-198. 1210-0668. DOI: https://doi.org/10.2478/enr-2018-0024
APA:
Mohamed, M., Hafez, H., Mohamed, H., Zenhom, N. (2018). STAT3 and Nrf2 pathways modulate the protective effect of verapamil on lung injury of diabetic rats. ENDOCRINE REGULATIONS, 52(4), 192-198. 1210-0668. DOI: https://doi.org/10.2478/enr-2018-0024
O vydaní:
Vydavateľ: Sciendo
Publikované: 22. 11. 2018