MicroRNA-219-5p functions as a tumor suppressor partially by targeting platelet-derived growth factor receptor alpha in colorectal cancer

In: NEOPLASMA, vol. 62, no. 6

G. Xiong - G. Zhang - Y. Xiao - B. Niu - H. Qiu - B. Wu - G. Lin - L. You - H. Shu

Detaily:

Rok, strany: 2015, 855 - 863

O článku:

Platelet-derived growth factor receptor (PDGFR) signaling pathway was involved in the progress of colorectal cancer (CRC). By using the bioinformatic system online, we found that PDGFRα is a potential target of miR-219-5p. However, the expression pattern and underlying mechanisms of miR-219-5p had not been elucidated in CRC. Herein, we first evaluated the expression of miR-219-5p in tumor tissues by real-time polymerase chain reaction. Next, we confirmed that PDGFRα is the target of miR-219-5p by using luciferase report. And then, we investigated the biological functions of miR-219-5p in vitro in cell proliferation and apoptosis as well as cell cycle by gain and loss of function strategies. Data shown that miR-219-5p is down-regulated in CRC tissues compared with the corresponding matched normal tissues. PDGFRα was a direct target of miR-219-5p. Overexpression of miR-219-5p could inhibit cell proliferation, promote cell apoptosis and induce cell cycle arrest at the G1 phase. Furthermore, miR-219-5p suppressed the activation of the phosphatidylinositol 3-kinase/Akt signaling pathway and downregulated G1 cell-cycle-related protein cyclin D1, cyclin-dependent kinase (CDK) 4, and CDK6. Taken together, our results demonstrate that miR-219-5p functions as a tumor suppressor partially by targeting PDGFRα in colorectal cancer. Keywords: microRNA, colorectal cancer, miR-219-5p, PDGFRα, PI3K/Akt.

Ako citovať:

ISO 690:

Xiong, G., Zhang, G., Xiao, Y., Niu, B., Qiu, H., Wu, B., Lin, G., You, L., Shu, H. 2015. MicroRNA-219-5p functions as a tumor suppressor partially by targeting platelet-derived growth factor receptor alpha in colorectal cancer. In NEOPLASMA, vol. 62, no.6, pp. 855-863. 0028-2685.

APA:

Xiong, G., Zhang, G., Xiao, Y., Niu, B., Qiu, H., Wu, B., Lin, G., You, L., Shu, H. (2015). MicroRNA-219-5p functions as a tumor suppressor partially by targeting platelet-derived growth factor receptor alpha in colorectal cancer. NEOPLASMA, 62(6), 855-863. 0028-2685.