Electronic Library of Scientific Literature - © Academic Electronic Press
Original articles
Volume 37 / No. 1 / 2004
Quintanar JL,
Salinas E, Chávez-Morales R-M, Quintanar-Stephano A (Aguascalientes, México)
Pituitary synaptic protein SNAP-25 sensitive to GnRH is
necessary for LH release
She-Fang Ye, Wakame
K, Ichimura K, Matsuzaki S (Mibu and Sapporo, Japan)
Amelioration by active hexose correlated compound of endocrine
disturbances induced by oxidative stress in the rat
Gregoraszczuk EL,
Ptak A, Wojtowicz AK, Gorska T, Nowak KW (Krakow and Poznan, Poland)
Estrus cycle-dependent action of leptin on basal and GH or IGF-I stimulated
steroid secretion by whole porcine follicles
Review
Orlowska-Majdak M
(Lodz, Poland)
Effect of excitatory amino acids on the activity of vasopressinergic and
oxytocinergic neurons
Nezbedova P, Brtko
J (Bratislava, Slovakia)
1a,25-dihydroxyvi tamin d3 inducible transcription factor and its
role in the vitamin d action
Book Reviews
Endocrine Regulations (since 1967 to 1990 Endocrinologia Experimentalis) is an international journal on experimental and clinical endocrinology edited quarterly in English by care of the Institute of Experimental Endocrinology, Slovak Academy of Sciences (Bratislava, Slovakia) and published by the Slovak Academic Press (Bratislava, Slovakia).
This journal aims to publish original manuscripts or minireviews on experimental and clinical endocrinology and diabetes.
The submission of a manuscript to Endocrine Regulations implies that it has not been previously published or is not being submitted for publication elsewhere and that the manuscript has been approved by all authors who are ready to take public responsibility for the content.
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A.Journal Articles:
Itoh M, Okugawa T, Shiratori N, Ohashi H: Treatment with triiodothyronine (T3) against multinodular goiter fails to prevent the onset of Graves disease. Endocrine Regul 29, 151-156, 1995
B. Book Chapters:
Mornex R, Orgiazzi JJ: Hyperthyroidism. In: The Thyroid Gland (Ed. M de Visscher), pp. 279-362, Raven Press, New York 1980
C. Books:
Podoba J: Endemic goiter in Slovakia. VEDA, Bratislava, 1962
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J. Luis Quintanar1, Eva Salinas2, Rosa María Chávez-Morales1, Andrés Quintanar-Stephano1
1 Laboratory of Neurophysiology, Department of Physiology and Pharmacology and 2Laboratory of Immunology, Department of Microbiology. Centro de Ciencias Básicas. Universidad Autónoma de Aguascalientes, Aguascalientes, México, E-mail: jlquinta@correo.uaa.mx
Objective. The protein SNAP-25 is located in the
plasma membrane and is known to participate in hormone exocytosis process. In
the present work we studied the role of SNAP-25 on LH secretion in permeabilized
adenohypophyseal cultured cells. The question of whether GnRH regulates SNAP-25
expression in adenohypophyseal cultured cells and in the adenohypophyses in vivo
was also investigated.
Methods. In digitonin-permeabilized cells incubated with anti-SNAP-25,
stimulated LH secretion with Ca2+ was analysed. The presence and expression of
SNAP-25 in adenohypophyseal cultured cells incubated with GnRH and in
adenohypophyses of orchidectomized rats with GnRH administration was studied by
immunochemistry and immunoblotting.
Results. Immunochemical study revealed that SNAP-25 was present in cultured
adenohypophyseal cells and in adenohypophysis of orchidectomized rats both with
GnRH treatment. We found that LH secretion can be blocked by antobodies raised
against SNAP-25 in permeabilized cells. Likewise, GnRH administration induced
a significant decrease of SNAP-25 expression in cultured adenohypophyseal
cells and in adenohypophysis of orchidectomized rats.
Conclusion. Our study showed that SNAP-25 is present in adenohypophyses in vitro
as well as in vivo and that is involved in LH release and that GnRH can modify
its expression.
Key words: Gonadotrophs – Immunohistochemistry – Immunoblot
– Permeabilized cells – Digitonin
ENDOCRINE REGULATIONS, Vol. 38, 1–6, 2004
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She-Fang Ye, K. Wakame1, K. Ichimura, S. Matsuzaki
Department of Biochemistry, Dokkyo University School of Medicine, Mibu, Tochigi, 321- 0293 Japan; 1Amino U P Chemical, Ltd, Sapporo, 0049-0839 Japan, E-mail: matuzaki@dokkyomed.ac.jp
Objective. Active hexose correlated compound (AHCC),
an extract derived from fungi of Basidiomycetes family, has been found to be
a potent antioxidant. Since the secretion of some hormones can be affected
by reactive oxygen species, the objective of this study was to examine how
ferric nitrilotriacetate (FeNTA), which generates hydroxyl radicals in vivo,
modulates the hormone secretion and the effects of AHCC.
Methods. AHCC at 3 % in drinking water was given to male rats for one week,
and the animals were decapitated at different time intervals after the treatment
with FeNTA intraperitoneally. Serum levels of hormones (corticosterone,
testosterone, thyroxine and triiodothyronine), adrenal ascorbic acid as well as
changes in hepatic oxidative status were evaluated by immunoassay and
spectrometry.
Results. Serum corticosterone levels increased significantly following FeNTA
treatment, while AHCC reduced the increased levels to normal. Adrenal ascorbic
acid levels that reflect ACTH secretion, were decreased by FeNTA and restored to
normal by AHCC. Serum levels of testosterone and thyroxine (T4) decreased
rapidly after FeNTA treatment, while AHCC pretreatment prevented this fall.
Serum triiodothyroxine (T3) levels remained unchanged either by FeNTA or AHCC
treatment. The hepatic oxidized glutathione, glutathione-related enzymes and
also serum lipid peroxide were greatly enhanced after FeNTA treatment. All of
these changes were restored to normal by AHCC pretreatment.
Conclusion. FeNTA induces various endocrine disorders and AHCC ameliorates these
effects by acting as an antioxidant.
Key words: Oxidative stress – Corticosterone – Testosterone
– Thyroid hormone – Fungi extract – Endocrine disorders
ENDOCRINE REGULATIONS, Vol. 38, 7–13, 2004
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Ewa L. Gregoraszczuk1, Anna Ptak1, Anna K.Wojtowicz1, Tatiana Gorska2, Krzysztof W. Nowak2
1 Laboratory of Physiology and Toxicology of Reproduction, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Krakow, Poland; 2 Department of Animal Physiology and Biochemistry, August Cieszkowski University of Agriculture, Poznan, Poland, E-mail: greg@zuk.iz.uj.edu.pl
Objective. To determine the levels of leptin in the
follicular fluid and using culture of whole ovarian follicles, to test the
hypothesis that leptin may directly influence GH and IGF-I stimulated ovarian
function.
Methods. Porcine follicles were recovered from ovaries during early, middle, and
preovulatory stage of the follicular phase of the estrus cycle. They were
cultured in the presence of the recombinant ovine leptin (oLEP) added either
alone or with oGH or hIGF-I. Steroid concentrations in the media were determined
after 48 h of culture
Results. The respective values for leptin in follicular fluid from small, medium
and large follicles were 1.98, 2.18 and 1.96 ng/ml, respectively. Leptin added
alone at a dose of 2 ng/ml had no effect on basal steroid secretion by
small and medium follicles. However, in small follicles a synergic action
of GH and IGF-I was noted. Leptin did not influence the secretion of
progesterone by follicles collected during the early and middle follicular
phases. In preovulatory follicles, leptin added alone to the culture media
caused a decrease in basal estradiol secretion with a concomitant
increase in progesterone secretion. Moreover, it acted synergistically with
IGF-I and GH causing further stimulation of progesterone secretion.
Conclusions. The presented data show a direct, maturation dependent action
of leptin on GH and IGF-I stimulated follicular steroidogenesis. During
follicular growth they acted synergistically with GH and IGF-I in estradiol
production, while in preovulatory follicles, they acted with both investigated
hormones in luteinization process, which starts before follicular disruption.
Key words: Leptin – Estrus cycle – GH – IGF-I – Steroid
secretion – Porcine follicles
ENDOCRINE REGULATIONS, Vol. 38, 15–21, 2004
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Monika Orlowska-Majdak
Department of Experimental and Clinical Physiology, Institute of Physiology and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland, E-mail: morlowska@zdn.am.lodz.pl
Objectives. A few compounds function as the excitatory
amino acid (EAA) transmitters in the central nervous system (CNS), but glutamate
(Glu) is the most important. Data on Glu participation in the control of
vasopressinergic (AVP-ergic) and oxytocinergic (OXT-ergic) neuronal activity
have been collected mainly on the basis of observations of hypothalamic
AVP-ergic and OXT-ergic neurons. In vivo and in vitro experiments have
demonstrated that Glu enhances bioelectric activity of the aforementioned
neurons and increases AVP and OXT release. However, inhibitory effect of Glu on
AVP-ergic neurons, mediated by local GABA-ergic interneurons, is also possible.
Both ionotropic and metabotropic receptors participate in EAA effect on
AVP-ergic and OXT-ergic neurons. EAA involvement in AVP and OXT release after
osmotic stimuli and in OXT release during the milk ejection reflex has been
demonstrated. Recent findings demonstrated that EAA enhanced AVP release into
the extracellular fluid of hippocampus in the rabbit.
Key words: Vasopressinergic and oxytocinergic neurons – Excitatory
amino acids – Hypothalamus – Hippocampus – Glutamate
ENDOCRINE REGULATIONS, Vol. 38, 23–28, 2004
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P. Nezbedova, J. Brtko
Institute of Experimental Endocrinology, Slovak Academy of Sciences, 833 06 Bratislava, Slovakia
Objectives. Vitamin D is considered
multifunctional steroid hormone that modulates calcium homeostasis through
actions predominantly in kidney, bone and the intestinal tract. Nuclear vitamin
D receptor (VDR) is a specific nuclear protein, a member of
steroid hormone receptor superfamily. The amino acid sequence of the VDR shows
a significant homology with other members of the nuclear hormone receptor
superfamily, including receptors for glucocorticoids (GR), oestrogen (ER),
androgen (AR), progesteron (PR), thyroid hormone (T3R), retinoic acid
(RAR), retinoid X (RXR) and over 150 orphan receptors. VDR is known to
mediate the pleiotropic biological actions of 1a,25-dihydroxyvitamin
D3 through its ability to modulate the expression of target genes. VDR upon
binding 1a,25-dihydroxyvitamin D3
regulates specific gene transcription predominantly by binding as
a heterodimer with the retinoid X receptor (RXR) to DNA enhancer
sequence, termed the vitamin D-responsive element (VDRE) that is present within
the promoter region of vitamin D-controlled genes. The VDR has been shown to
associate with several additional molecules to form the active transcriptional
complex required for gene regulation. The regulation of this ligand-activated
cellular transcription factor occurs at both transcriptional and
posttranslational levels. This article summarizes a variety of effects of 1a,25-dihydroxyvitamin
D3, acting through its cognate nuclear receptor, and its use in
chemotherapy and chemoprevention of cancer.
Key words: Vitamin D3 – Nuclear receptors – Mechanism of
action – Gene expression
ENDOCRINE REGULATIONS, Vol. 38, 29–38, 2004
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