Electronic Library of Scientific Literature - © Academic Electronic Press
Volume 37 / No. 1 / 2004
Salinas E, Chávez-Morales R-M, Quintanar-Stephano A (Aguascalientes, México)
Pituitary synaptic protein SNAP-25 sensitive to GnRH is necessary for LH release
She-Fang Ye, Wakame
K, Ichimura K, Matsuzaki S (Mibu and Sapporo, Japan)
Amelioration by active hexose correlated compound of endocrine disturbances induced by oxidative stress in the rat
Ptak A, Wojtowicz AK, Gorska T, Nowak KW (Krakow and Poznan, Poland)
Estrus cycle-dependent action of leptin on basal and GH or IGF-I stimulated steroid secretion by whole porcine follicles
Effect of excitatory amino acids on the activity of vasopressinergic and oxytocinergic neurons
Nezbedova P, Brtko
J (Bratislava, Slovakia)
1a,25-dihydroxyvi tamin d3 inducible transcription factor and its role in the vitamin d action
Endocrine Regulations (since 1967 to 1990 Endocrinologia Experimentalis) is an international journal on experimental and clinical endocrinology edited quarterly in English by care of the Institute of Experimental Endocrinology, Slovak Academy of Sciences (Bratislava, Slovakia) and published by the Slovak Academic Press (Bratislava, Slovakia).
This journal aims to publish original manuscripts or minireviews on experimental and clinical endocrinology and diabetes.
The submission of a manuscript to Endocrine Regulations implies that it has not been previously published or is not being submitted for publication elsewhere and that the manuscript has been approved by all authors who are ready to take public responsibility for the content.
All materials relating to human investigation will be published upon the understanding that design of the work has been approved by the local Ethical Committee or that it conforms to ethical guidelines of the Declaration of Helsinki. The animal experiments should state the conformance to guidelines on animal care.
Manuscripts in triplicate with three sets of illustrations (of which one is an original) should be sent to:
Richard Kvetnansky, Ph.D., Dr.Sc., Chief Editor,
Institute of Experimental Endocrinology,
Vlárska 3, 833 06 Bratislava, Slovakia
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Types of manuscripts. Standard original papers should contain following sections: * Title, * Abstract (divided into sections Objective, Methods, Results, Conclusions), * Key Words, * Introduction, * Materials and Methods (in clinical papers this section should read * Subjects and Methods), * Results, * Discussion, (* Acknowledgements), * References. There is no length limit for these papers.
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Itoh M, Okugawa T, Shiratori N, Ohashi H: Treatment with triiodothyronine (T3) against multinodular goiter fails to prevent the onset of Graves disease. Endocrine Regul 29, 151-156, 1995
B. Book Chapters:
Mornex R, Orgiazzi JJ: Hyperthyroidism. In: The Thyroid Gland (Ed. M de Visscher), pp. 279-362, Raven Press, New York 1980
Podoba J: Endemic goiter in Slovakia. VEDA, Bratislava, 1962
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J. Luis Quintanar1, Eva Salinas2, Rosa María Chávez-Morales1, Andrés Quintanar-Stephano1
1 Laboratory of Neurophysiology, Department of Physiology and Pharmacology and 2Laboratory of Immunology, Department of Microbiology. Centro de Ciencias Básicas. Universidad Autónoma de Aguascalientes, Aguascalientes, México, E-mail: email@example.com
Objective. The protein SNAP-25 is located in the
plasma membrane and is known to participate in hormone exocytosis process. In
the present work we studied the role of SNAP-25 on LH secretion in permeabilized
adenohypophyseal cultured cells. The question of whether GnRH regulates SNAP-25
expression in adenohypophyseal cultured cells and in the adenohypophyses in vivo
was also investigated.
Methods. In digitonin-permeabilized cells incubated with anti-SNAP-25, stimulated LH secretion with Ca2+ was analysed. The presence and expression of SNAP-25 in adenohypophyseal cultured cells incubated with GnRH and in adenohypophyses of orchidectomized rats with GnRH administration was studied by immunochemistry and immunoblotting.
Results. Immunochemical study revealed that SNAP-25 was present in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats both with GnRH treatment. We found that LH secretion can be blocked by antobodies raised against SNAP-25 in permeabilized cells. Likewise, GnRH administration induced a significant decrease of SNAP-25 expression in cultured adenohypophyseal cells and in adenohypophysis of orchidectomized rats.
Conclusion. Our study showed that SNAP-25 is present in adenohypophyses in vitro as well as in vivo and that is involved in LH release and that GnRH can modify its expression.
Key words: Gonadotrophs – Immunohistochemistry – Immunoblot – Permeabilized cells – Digitonin
ENDOCRINE REGULATIONS, Vol. 38, 1–6, 2004
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She-Fang Ye, K. Wakame1, K. Ichimura, S. Matsuzaki
Department of Biochemistry, Dokkyo University School of Medicine, Mibu, Tochigi, 321- 0293 Japan; 1Amino U P Chemical, Ltd, Sapporo, 0049-0839 Japan, E-mail: firstname.lastname@example.org
Objective. Active hexose correlated compound (AHCC),
an extract derived from fungi of Basidiomycetes family, has been found to be
a potent antioxidant. Since the secretion of some hormones can be affected
by reactive oxygen species, the objective of this study was to examine how
ferric nitrilotriacetate (FeNTA), which generates hydroxyl radicals in vivo,
modulates the hormone secretion and the effects of AHCC.
Methods. AHCC at 3 % in drinking water was given to male rats for one week, and the animals were decapitated at different time intervals after the treatment with FeNTA intraperitoneally. Serum levels of hormones (corticosterone, testosterone, thyroxine and triiodothyronine), adrenal ascorbic acid as well as changes in hepatic oxidative status were evaluated by immunoassay and spectrometry.
Results. Serum corticosterone levels increased significantly following FeNTA treatment, while AHCC reduced the increased levels to normal. Adrenal ascorbic acid levels that reflect ACTH secretion, were decreased by FeNTA and restored to normal by AHCC. Serum levels of testosterone and thyroxine (T4) decreased rapidly after FeNTA treatment, while AHCC pretreatment prevented this fall. Serum triiodothyroxine (T3) levels remained unchanged either by FeNTA or AHCC treatment. The hepatic oxidized glutathione, glutathione-related enzymes and also serum lipid peroxide were greatly enhanced after FeNTA treatment. All of these changes were restored to normal by AHCC pretreatment.
Conclusion. FeNTA induces various endocrine disorders and AHCC ameliorates these effects by acting as an antioxidant.
Key words: Oxidative stress – Corticosterone – Testosterone – Thyroid hormone – Fungi extract – Endocrine disorders
ENDOCRINE REGULATIONS, Vol. 38, 7–13, 2004
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Ewa L. Gregoraszczuk1, Anna Ptak1, Anna K.Wojtowicz1, Tatiana Gorska2, Krzysztof W. Nowak2
1 Laboratory of Physiology and Toxicology of Reproduction, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Krakow, Poland; 2 Department of Animal Physiology and Biochemistry, August Cieszkowski University of Agriculture, Poznan, Poland, E-mail: email@example.com
Objective. To determine the levels of leptin in the
follicular fluid and using culture of whole ovarian follicles, to test the
hypothesis that leptin may directly influence GH and IGF-I stimulated ovarian
Methods. Porcine follicles were recovered from ovaries during early, middle, and preovulatory stage of the follicular phase of the estrus cycle. They were cultured in the presence of the recombinant ovine leptin (oLEP) added either alone or with oGH or hIGF-I. Steroid concentrations in the media were determined after 48 h of culture
Results. The respective values for leptin in follicular fluid from small, medium and large follicles were 1.98, 2.18 and 1.96 ng/ml, respectively. Leptin added alone at a dose of 2 ng/ml had no effect on basal steroid secretion by small and medium follicles. However, in small follicles a synergic action of GH and IGF-I was noted. Leptin did not influence the secretion of progesterone by follicles collected during the early and middle follicular phases. In preovulatory follicles, leptin added alone to the culture media caused a decrease in basal estradiol secretion with a concomitant increase in progesterone secretion. Moreover, it acted synergistically with IGF-I and GH causing further stimulation of progesterone secretion.
Conclusions. The presented data show a direct, maturation dependent action of leptin on GH and IGF-I stimulated follicular steroidogenesis. During follicular growth they acted synergistically with GH and IGF-I in estradiol production, while in preovulatory follicles, they acted with both investigated hormones in luteinization process, which starts before follicular disruption.
Key words: Leptin – Estrus cycle – GH – IGF-I – Steroid secretion – Porcine follicles
ENDOCRINE REGULATIONS, Vol. 38, 15–21, 2004
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Department of Experimental and Clinical Physiology, Institute of Physiology and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland, E-mail: firstname.lastname@example.org
Objectives. A few compounds function as the excitatory
amino acid (EAA) transmitters in the central nervous system (CNS), but glutamate
(Glu) is the most important. Data on Glu participation in the control of
vasopressinergic (AVP-ergic) and oxytocinergic (OXT-ergic) neuronal activity
have been collected mainly on the basis of observations of hypothalamic
AVP-ergic and OXT-ergic neurons. In vivo and in vitro experiments have
demonstrated that Glu enhances bioelectric activity of the aforementioned
neurons and increases AVP and OXT release. However, inhibitory effect of Glu on
AVP-ergic neurons, mediated by local GABA-ergic interneurons, is also possible.
Both ionotropic and metabotropic receptors participate in EAA effect on
AVP-ergic and OXT-ergic neurons. EAA involvement in AVP and OXT release after
osmotic stimuli and in OXT release during the milk ejection reflex has been
demonstrated. Recent findings demonstrated that EAA enhanced AVP release into
the extracellular fluid of hippocampus in the rabbit.
Key words: Vasopressinergic and oxytocinergic neurons – Excitatory amino acids – Hypothalamus – Hippocampus – Glutamate
ENDOCRINE REGULATIONS, Vol. 38, 23–28, 2004
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P. Nezbedova, J. Brtko
Institute of Experimental Endocrinology, Slovak Academy of Sciences, 833 06 Bratislava, Slovakia
Objectives. Vitamin D is considered
multifunctional steroid hormone that modulates calcium homeostasis through
actions predominantly in kidney, bone and the intestinal tract. Nuclear vitamin
D receptor (VDR) is a specific nuclear protein, a member of
steroid hormone receptor superfamily. The amino acid sequence of the VDR shows
a significant homology with other members of the nuclear hormone receptor
superfamily, including receptors for glucocorticoids (GR), oestrogen (ER),
androgen (AR), progesteron (PR), thyroid hormone (T3R), retinoic acid
(RAR), retinoid X (RXR) and over 150 orphan receptors. VDR is known to
mediate the pleiotropic biological actions of 1a,25-dihydroxyvitamin
D3 through its ability to modulate the expression of target genes. VDR upon
binding 1a,25-dihydroxyvitamin D3
regulates specific gene transcription predominantly by binding as
a heterodimer with the retinoid X receptor (RXR) to DNA enhancer
sequence, termed the vitamin D-responsive element (VDRE) that is present within
the promoter region of vitamin D-controlled genes. The VDR has been shown to
associate with several additional molecules to form the active transcriptional
complex required for gene regulation. The regulation of this ligand-activated
cellular transcription factor occurs at both transcriptional and
posttranslational levels. This article summarizes a variety of effects of 1a,25-dihydroxyvitamin
D3, acting through its cognate nuclear receptor, and its use in
chemotherapy and chemoprevention of cancer.
Key words: Vitamin D3 – Nuclear receptors – Mechanism of action – Gene expression
ENDOCRINE REGULATIONS, Vol. 38, 29–38, 2004
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Electronic Library of Scientific Literature - © Academic Electronic Press