The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c

Rok, strany: 2013, 189 - 200

We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix[6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at an air-water interface and complexes in mixed monolayers contained DPhPC. Calixarenes increase the elastic moduli of lipid monolayers as well as BLM. Cytochrome c (cyt c) specifically binds to CX by incorporation of the amino groups of lysine residues at the protein surface. This binding affected the physical properties of CX monolayers depending on their initial surface pressure. Addition of cyt c into the water subphase induced increase of surface pressure of CX monolayers at relatively low initial pressure (15 mN/m) when monolayer was in liquid expanded state (LE). This may be due interaction of positively charged cyt c with negatively charged carboxylic groups of CX and also by its penetration into the air-water interface. However, much subtle changes were observed for higher initial surface pressure (20 and 35 mN/m) when monolayer is in liquid condensed (LC) and solid (S) state, respectively. Lysine induced substantially lower changes in surface pressure in comparison with that of cyt c.

ISO 690:

Vitovič, P., Šubjaková, V., Hianik, T. 2013. The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c. In General Physiology and Biophysics, vol. 32, no.2, pp. 189-200. 0231-5882.

APA:

Vitovič, P., Šubjaková, V., Hianik, T. (2013). The physical properties of lipid monolayers and bilayers containing calixarenes sensitive to cytochrome c. General Physiology and Biophysics, 32(2), 189-200. 0231-5882.