Transport of lithium across the lamprey (Lampetra fluviatilis) erythrocyte membrane

Rok, strany: 2008, 284 - 290

Lithium, capable of replacing Na⁺ in various membrane transport processes, was used to investigate Na⁺ transport pathways across the lamprey erythrocytes membrane. The values of Li⁺ influxes have ranged from 8 to 24 mmol/l cells/h. Intracellular accumulation of Li⁺ was associated with loss of cellular Na⁺, the value of which was less than the value of Li⁺ influx. Both Li⁺ influx and Na⁺ efflux were partially inhibited by amiloride. The amiloride-sensitive Li⁺ influx was considerably stimulated by hyperosmotic cell shrinkage. The treatment of lamprey erythrocytes with blockers of protein phosphatases (fluoride and cantharidin) also resulted in a considerable increase in Li⁺ accumulation within the cells. No significant difference was observed between the values of Li⁺ and Na⁺ (²²Na) influxes measured in red cells incubated simultaneously in isotonic LiCl and NaCl media (9.2 ± 2.1 and 7.8 ± 1.3 mmol/l cells/h, respectively). In hypo- and hypertonic media, however, the rate of Na⁺ influx in lamprey erythrocytes was approximately twice higher as compared to the rate of Li⁺ influx, what was determined by the difference in the amiloride-sensitive components. In acidified lamprey erythrocytes (intracellular pH 6.0) Li⁺ and Na⁺ influxes were considerably increased due to activation of amiloride-sensitive Na⁺/H⁺ (Li⁺/H⁺) exchange mechanism, although the activity of Na⁺/H⁺ exchange was much greater than that of Li⁺/H⁺ exchange. The data obtained confirm the hypothesis on the presence of two amiloride-sensitive systems of Na⁺ transport in the lamprey red blood cells.

ISO 690:

Gusev, G., Agalakova, N., Ivanova, T. 2008. Transport of lithium across the lamprey (Lampetra fluviatilis) erythrocyte membrane. In General Physiology and Biophysics, vol. 27, no.4, pp. 284-290. 0231-5882.

APA:

Gusev, G., Agalakova, N., Ivanova, T. (2008). Transport of lithium across the lamprey (Lampetra fluviatilis) erythrocyte membrane. General Physiology and Biophysics, 27(4), 284-290. 0231-5882.