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Effect of 7-nitroindazole on the expression of intracellular calcium channels in the kidney of spontaneously hypertensive rats

In: General Physiology and Biophysics, vol. 28, no. 3
Barbora Sedlakova - Sona Cacanyiova - Karol Ondrias - Frantisek Kristek - Olga Krizanova
Detaily:
Rok, strany: 2009, 225 - 232
O článku:
The spontaneously hypertensive rats (SHR) were fed with nitric oxide synthase (NOS) blocker 7-nitroindazole (7-NI, 10 mg/kg/day) for 6 weeks and an expression of intracellular calcium channels, SERCA and proapoptotic agents was evaluated in kidney. Treatment of rats with 7-NI resulted in a significant increase in mRNA and protein levels of the IP3 receptors type 1 and type 2, while mRNA levels of the IP3 receptor type 3 remained unchanged. The mRNA of other intracellular calcium channels, ryanodine receptors type 1 and type 2 was also upregulated by 7-NI treatment. Gene expression of the SERCA2a, calcium pump responsible for loading intracellular stores with calcium, revealed increased gene expression due to 7-NI as well. Interestingly, proapoptotic agents caspase 3 and Bax were also upregulated by the 7-NI treatment. These results may indicate that nNOS blocker 7-NI modifies intracellular calcium transport system, which may have impact on altered calcium handling and regulation of various metabolic pathways.
Ako citovať:
ISO 690:
Sedlakova, B., Cacanyiova, S., Ondrias, K., Kristek, F., Krizanova, O. 2009. Effect of 7-nitroindazole on the expression of intracellular calcium channels in the kidney of spontaneously hypertensive rats. In General Physiology and Biophysics, vol. 28, no.3, pp. 225-232. 0231-5882.

APA:
Sedlakova, B., Cacanyiova, S., Ondrias, K., Kristek, F., Krizanova, O. (2009). Effect of 7-nitroindazole on the expression of intracellular calcium channels in the kidney of spontaneously hypertensive rats. General Physiology and Biophysics, 28(3), 225-232. 0231-5882.