Influence of sodium nitroprusside on human erythrocyte membrane water permeability: an NMR study

Rok, strany: 2010, 373 - 380

Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'2a in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has been measured. The observed T'2a time-course was analyzed in terms of the two mechanisms by which released •NO affects T'2a. These are, respectively, enhancement of the intracellular water proton intrinsic NMR relaxation rate 1/T2a by paramagnetism of •NO subsequently bonded to iron atoms of intracellular deoxyhemoglobin, and suppression of diffusional water permeability Pd as a consequence of nitrosylation of aquaporin-1 (AQP1) channel Cys189, either by direct reaction with •NO or with one of the •NO oxidation products, such as N2O3. The bound •NO on the Cys189 thiol residue appears to impose a less efficient barrier to water permeation through AQP1 than the larger carboxyphenylmercuryl residue from p-chloromercuribenzoate. The effect of •NO on Pd is discussed in terms of NO-induced vasodilation.

ISO 690:

Lahajnar, G., Sobotič, B., Sepe, A., Jazbinšek, V., Pečar, S. 2010. Influence of sodium nitroprusside on human erythrocyte membrane water permeability: an NMR study. In General Physiology and Biophysics, vol. 29, no.4, pp. 373-380. 0231-5882.

APA:

Lahajnar, G., Sobotič, B., Sepe, A., Jazbinšek, V., Pečar, S. (2010). Influence of sodium nitroprusside on human erythrocyte membrane water permeability: an NMR study. General Physiology and Biophysics, 29(4), 373-380. 0231-5882.