Informácia o dokumente



In: General Physiology and Biophysics, vol. 30, no. 3
Petronela Dianišková - Jana Korduláková - Henrieta Škovierová - Devinder Kaur - Mary Jackson - Patrick Brennan - Katarína Mikušová

Investigation of ABC transporter from mycobacterial arabinogalactan biosynthetic cluster

Detaily:

Rok, strany: 2011, 239 - 250

O článku:

Two genes from the "mycobacterial arabinogalactan biosynthetic cluster" spanning the region from Rv3779 to Rv3809c in the genome of Mycobacterium tuberculosis H37Rv were annotated as possible components of the ATP-binding cassette transporter. Rv3781 encodes a nucleotide-binding domain and Rv3783 determines production of a membrane-spanning domain. We have examined possible roles of these genes in mycobacterial cell wall biosynthesis through inactivation of their respective orthologs in Mycobacterium smegmatis mc2155, phenotypic characterization of the mutant strains via metabolic labeling with [U-14C]-glucose, cell-free reactions with UDP-[U-14C]-galactose monitoring galactan build-up and transcriptional analysis. Several lines of evidence suggest that this ABC transporter is involved in biosynthesis of arabinogalactan, although more investigation is needed to establish its precise role or the transported substrate.

Ako citovať:

ISO 690:
Dianišková, P., Korduláková, J., Škovierová, H., Kaur, D., Jackson, M., Brennan, P., Mikušová, K. 2011. Investigation of ABC transporter from mycobacterial arabinogalactan biosynthetic cluster. In General Physiology and Biophysics, vol. 30, no.3, pp. 239-250. 0231-5882.

APA:
Dianišková, P., Korduláková, J., Škovierová, H., Kaur, D., Jackson, M., Brennan, P., Mikušová, K. (2011). Investigation of ABC transporter from mycobacterial arabinogalactan biosynthetic cluster. General Physiology and Biophysics, 30(3), 239-250. 0231-5882.