Proteasome inhibition leads to altered signaling in the proteome of cisplatin-resistant human ovarian carcinoma cell line

Rok, strany: 2013, 627 - 634

To address a precise view into molecular mechanisms of apoptotic signaling pathways after single- or combinatory treatments with specific NF-κB- or proteasome inhibitors and/or cisplatin (CDDP), flow cytometry and western blotting of the cell proteome in human ovarian chemosensitive- and CDDP-resistant cell lines were used. We report here that proteasome inhibition (but not NF-κB inhibition) caused marked alterations in the cell proliferation and cell cycle, as well as in the levels of signaling anti- and pro-apoptotic proteins PARP, NF-κB, IκB-α, Bcl-2, Bax, and lysosome-associated LAMP-1 and ATP-7B molecules in particular proteome fractions. These findings refer to the possibility of regulation of CDDP resistance, inclusive the capacity of lysosomes to export CDDP in certain human ovarian cancer cells by proteasome inhibition. Keywords: cisplatin resistance, cell proteome, NF-κB- and proteosome inhibition, flow cytometry, western blotting

ISO 690:

Duraj, J., Pastorek, M., Vitkovska, J., Cholujova, D., Gronesova, P., Hunakova, L., Sedlak, J. 2013. Proteasome inhibition leads to altered signaling in the proteome of cisplatin-resistant human ovarian carcinoma cell line. In NEOPLASMA, vol. 60, no.6, pp. 627-634. 0028-2685.

APA:

Duraj, J., Pastorek, M., Vitkovska, J., Cholujova, D., Gronesova, P., Hunakova, L., Sedlak, J. (2013). Proteasome inhibition leads to altered signaling in the proteome of cisplatin-resistant human ovarian carcinoma cell line. NEOPLASMA, 60(6), 627-634. 0028-2685.