The effect of prolactin and estrogen cross-talk on prolidase– dependent signaling in MCF-7 cells

Rok, strany: 2013, 355 - 363

Estrogen and prolactin play important role in mammary carcinogenesis. The present study was undertaken to evaluate the effect of prolactin and estrogen cross-talk on HIF-1 α level and expression of some HIF-1 α - dependent signaling proteins. Since up-regulation of prolidase activity inhibits HIF-1 α degradation, the enzyme was considered as an interface of estrogen/prolactin signaling. The experiments were performed on MCF-7 cells cultured with prolactin in the presence or absence of estradiol. It was found that in the presence of estradiol, prolactin inhibits prolidase activity and its down-stream signaling proteins: HIF-1α, mTOR, AKT and MAPK p-38, while in the absence of estradiol, an opposite effect was observed. These results suggest that prolactin/estrogen cross-talk exert beneficial effect on prolidase-dependent down regulation of HIF-1α. It suggests that dual action of prolactin and estrogen may be considered as a strategy in therapy of breast cancer. Keywords: Prolactin, estrogen, HIF-1 α, MCF-7, prolidase, cross-talk

ISO 690:

Surazynski, A., Miltyk, W., Wolczynski, S., Palka, J. 2013. The effect of prolactin and estrogen cross-talk on prolidase– dependent signaling in MCF-7 cells. In NEOPLASMA, vol. 60, no.4, pp. 355-363. 0028-2685.

APA:

Surazynski, A., Miltyk, W., Wolczynski, S., Palka, J. (2013). The effect of prolactin and estrogen cross-talk on prolidase– dependent signaling in MCF-7 cells. NEOPLASMA, 60(4), 355-363. 0028-2685.