Enhanced chemosensitivity to CPT-11 in colorectal carcinoma xenografts by small hairpin RNA interference targeting PLK1

In: NEOPLASMA, vol. 59, no. 6

P. Fan - S. Zhang - H. Tian - N. Yan - L. Dai - X. Zhang - L. Cheng - C. Li - Y. Li - X. Chen - G . Shi - Y . Yang - Y. Wei - H. Deng

Detaily:

Rok, strany: 2012, 676 - 684

O článku:

Commonly used drugs for the treatment of colon{} cancer patients like CPT-11 shows severe side effects or induces resistance in clinical settings. Thus, we analyzed a combination of PLK1 (polo-like kinase 1)-specific short hair RNA (shRNA), a potent tool to destroy mitosis in cancer cells, together with CPT-11 to enhance drug sensitivity. Cellular proliferation and apoptosis were determined in SW620 colorectal carcinoma cells. Knockdown of cellular PLK1 led to the decreased mRNA and PLK1 protein in RT-PCR and western blot assay. The viability declined (pnohistochemistry, accompanied with TUNEL assay. As we expect, the combination treatment delayed tumor growth (p of PLK1-specific shRNA interference with low-dose CPT-11 triggered a antitumor efficacy and represented a potential strategy to treat colon cancer. Keywords: CPT-11, drug resistance, SN-38, PLK1, RNA interference, colorectal carcinoma

Ako citovať:

ISO 690:

Fan, P., Zhang, S., Tian, H., Yan, N., Dai, L., Zhang, X., Cheng, L., Li, C., Li, Y., Chen, X., Shi, G., Yang, Y., Wei, Y., Deng, H. 2012. Enhanced chemosensitivity to CPT-11 in colorectal carcinoma xenografts by small hairpin RNA interference targeting PLK1. In NEOPLASMA, vol. 59, no.6, pp. 676-684. 0028-2685.

APA:

Fan, P., Zhang, S., Tian, H., Yan, N., Dai, L., Zhang, X., Cheng, L., Li, C., Li, Y., Chen, X., Shi, G., Yang, Y., Wei, Y., Deng, H. (2012). Enhanced chemosensitivity to CPT-11 in colorectal carcinoma xenografts by small hairpin RNA interference targeting PLK1. NEOPLASMA, 59(6), 676-684. 0028-2685.