Impact of blood processing on estimation of soluble HLA-G

Rok, strany: 2011, 337 - 342

HLA-G is a non-classical MHC class I antigen that functions as an immunomodulatory molecule. There are two forms of HLA-G antigens, soluble and membrane bound. Soluble HLA-G can be produced by translation of HLA-G transcripts (HLA-G5, -G6, -G7) and by shedding/proteolytic cleavage of membrane bound antigens (HLA-G1, -G2, -G3, -G4). Soluble as well as membrane bound HLA-G molecules have a direct inhibitory effect on immune responses. The relevance of soluble HLA-G in various pathologic conditions, such as transplantation, autoimmunity, infectious and malignant diseases, has been extensively investigated, however interpretation remains controversial.<br />In this work we analyzed the levels of sHLA-G (sHLA-G1 and HLA-G5) in different blood samples of healthy donors as serum, and blood plasma isolated using anti-coagulant EDTA and heparin, respectively. We found that the levels of sHLA-G (sHLA-G1 and HLA-G5) in blood plasma prepared with EDTA were significantly higher than those observed in plasma with heparin or in serum. Finally we detected the average levels of sHLA-G in females exceeded those of males. Keywords: soluble HLA-G, ELISA, plasma, serum

ISO 690:

Polăkovă, K., Bandĺ˝uchovă, E., Russ, G. 2011. Impact of blood processing on estimation of soluble HLA-G. In NEOPLASMA, vol. 58, no.4, pp. 337-342. 0028-2685.

APA:

Polăkovă, K., Bandĺ˝uchovă, E., Russ, G. (2011). Impact of blood processing on estimation of soluble HLA-G. NEOPLASMA, 58(4), 337-342. 0028-2685.