Effect of justicidin B – a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines

Rok, strany: 2011, 320 - 325

Justicidin B produced by genetically transformed cultures of Linum leonii was tested for cytotoxic activity and induction of apoptosis in MDA-MB-231 and MCF-7 breast cancer derived cell lines. The tested lignan evoked strong, concentration dependent cytotoxicity in both cell lines, whereby MCF-7 proved to be far more sensitive as compared to MDA-MB-231. The 24 h treatment of both cell lines increased the level of apoptotic DNA fragmentation; however the proapoptotic activity is completely inhibited if the cells are co-incubated with the non-selective pan-caspase inhibitor Boc-Asp(OMe)-fluoromethyl ketone (PCI), which implies that justicidin B, activates programmed cell death via caspase –dependent mechanisms. Exposure of MDA-MB-231 cells with justicidin B leads to concentration dependent decrease in the expression of NFkB; whereas the treatment of MCF-7, is consistent with strong increase in the expression of this transcription factor. Keywords: Justicidin B, cytotoxicity, apoptosis, NFkB, MCF-7, MDA-MB-231

ISO 690:

Momekov, G., Konstantinov, S., Dineva, I., Ionkova, I. 2011. Effect of justicidin B – a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines. In NEOPLASMA, vol. 58, no.4, pp. 320-325. 0028-2685.

APA:

Momekov, G., Konstantinov, S., Dineva, I., Ionkova, I. (2011). Effect of justicidin B – a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines. NEOPLASMA, 58(4), 320-325. 0028-2685.