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Expression of soluble TGF-β receptor II by recombinant Vaccinia virus enhances E7 specific immunotherapy of HPV16 tumors

In: NEOPLASMA, vol. 58, no. 3
K Zurkova - P Chlanda - Z Samkova - K Babiarova - L Kutinova - J Krystofova - P Hainz - S Nemeckova
Detaily:
Rok, strany: 2011, 181 - 188
O článku:
Therapeutic immunization with double recombinants of vaccinia virus (VACV) co-expressing sTβRII increased rejection of established TC-1 tumors in C57BL/6 mice in comparison with single recombinant expressing SigE7LAMP. Recombinant VACV derived from vaccination strain Praha expressed either the sTβRII (ectodomain) or chimeric protein fused to immunoglobulin Fc fragment (sTβRII-Fc-Jun) under control of two different promotors together with the immunogenic tumor associated antigen HPV16 E7 oncoprotein in a form of SigE7LAMP fusion molecule. The ability of soluble receptors to bind TGF-β in vitro was proved. Immunization of mice with double recombinant viruses and virus expressing SigE7LAMP only led to eliciting similar response of E7 specific CD8+ T cells as detected by IFN-γ ELISPOT. Keywords: HPV16, E7; vaccinia virus; TGF beta; TGF beta receptor II; tumor; immunity
Ako citovať:
ISO 690:
Zurkova, K., Chlanda, P., Samkova, Z., Babiarova, K., Kutinova, L., Krystofova, J., Hainz, P., Nemeckova, S. 2011. Expression of soluble TGF-β receptor II by recombinant Vaccinia virus enhances E7 specific immunotherapy of HPV16 tumors. In NEOPLASMA, vol. 58, no.3, pp. 181-188. 0028-2685.

APA:
Zurkova, K., Chlanda, P., Samkova, Z., Babiarova, K., Kutinova, L., Krystofova, J., Hainz, P., Nemeckova, S. (2011). Expression of soluble TGF-β receptor II by recombinant Vaccinia virus enhances E7 specific immunotherapy of HPV16 tumors. NEOPLASMA, 58(3), 181-188. 0028-2685.