In: NEOPLASMA, vol. 57, no. 6
M Adamkov - E Halasova - K Kajo - K Machalekova - D Vybohova - I Varga - J Rajcany
Detaily:
Rok, strany: 2010, 572 - 577
O článku:
The antiapoptotic protein survivin can be detected in most types of malignant tumors, but it is rarely expressed in corresponding normal adult tissues. Therefore, survivin appears to represent a promising diagnostic biomarker. We examined survivin expression in 13 cases of normal breast tissue, 38 cases of fibroadenomas and 80 cases of breast carcinomas by immunohistochemical staining using anti-survivin antibody (DAKO, Clone 12C4). In each section, the intensity of staining, percentage of labeled cells, and the subcellular location of survivin antigen were assessed. Survivin was detected in 4/13 cases of normal breast tissue (30.7%), in 28/38 cases of fibroadenomas (73.7%), and in 67/80 cases of carcinomas (83.8%). Normal breast tissue showed cytoplasmic positivity only. In fibroadenomas, 19 cases (50.0%) revealed cytoplasmic reaction, and in 9 cases (23.7%), small foci of cells with combined nuclear and cytoplasmic location were identified. In carcinomas, cytoplasmic staining was found in 12/80 cases (15.0%), nuclear staining in 10/80 cases (12.5%), and combined cytoplasmic and nuclear staining in 45/80 cases (56.3%). Subcellular location of survivin between benign and malignant lesions revealed significant differences (p<0,001). <br />Our findings point at practical use of survivin detection. We confirm the importance of nuclear staining of survivin antigen in breast carcinoma, which seems to be a notable diagnostic marker for estimation of the degree of neoplasia.<br /> Keywords: breast, fibroadenoma, carcinoma, survivin, biomarker
Ako citovať:
ISO 690:
Adamkov, M., Halasova, E., Kajo, K., Machalekova, K., Vybohova, D., Varga, I., Rajcany, J. 2010. Survivin: a promising biomarker in breast carcinoma. In NEOPLASMA, vol. 57, no.6, pp. 572-577. 0028-2685.
APA:
Adamkov, M., Halasova, E., Kajo, K., Machalekova, K., Vybohova, D., Varga, I., Rajcany, J. (2010). Survivin: a promising biomarker in breast carcinoma. NEOPLASMA, 57(6), 572-577. 0028-2685.