Identification of differential proteins in colon cancer SW480 cells with HIF1-α silence by proteome analysis

Rok, strany: 2010, 299 - 305

Hypoxia is one of the basic characteristics of the colorectal cancer and HIF1 plays a central role in tumor hypoxia adaptation and controls the expression of a variety of genes. But it had not reached a comprehensive understanding of them in colorectal cancer in hypoxia. This study silenced HIF1-α, which is the regulatory subunit of HIF1, and established two dimensional gel electrophoresis profiles for human SW480siHIF1-α and SW480N cells to identified proteins with differential expressions. Ten down-regulated proteins and nine up-regulated proteins were identified by HPLC-Chip-MS/MS. Down-regulated proteins including PLD2, ANXA2. HIF1-α had a clear positive correlation with PLD2 and ANXA2 in colon adenocarcinoma samples. There proteins might have a direct or indirect contact HIF1-α, and play a critical role in apoptosis, signal transduction and hypoxia adaptation. Keywords: colorectal neoplasms; hypoxia inducible factor1-α; RNA interference; proteomics analysis; phospholipase D2; annexin A2

ISO 690:

Wu, X., Fu, Z., Wang, X., Shen, W. 2010. Identification of differential proteins in colon cancer SW480 cells with HIF1-α silence by proteome analysis. In NEOPLASMA, vol. 57, no.4, pp. 299-305. 0028-2685.

APA:

Wu, X., Fu, Z., Wang, X., Shen, W. (2010). Identification of differential proteins in colon cancer SW480 cells with HIF1-α silence by proteome analysis. NEOPLASMA, 57(4), 299-305. 0028-2685.