Hypermethylation of the GSTP1 promoter region in breast cancer is associated with prognostic clinicopathological parameters

Rok, strany: 2010, 35 - 40

Breast cancer is one of the most common cancer affecting women and the recent research is focused on identifying new genetic and epigenetic prognostic and predictive factors. Glutathione S-transferase P1 (GSTP1) is a biotransformation enzyme expressed in normal breast epithelial cells which can be epigenetically inactivated in breast cancer. We have shown, that application of nested two-stage methylation-specific PCR (MSP) is a suitable method for analysis of epigenetically silenced GSTP1 in formalin-fixed paraffin-embedded (FFPE) tissues from breast cancer patients. Of 45 breast tumors, 11 (24, 4%) were found to have methylated GSTP 1 promoter region. We were able to demonstrate the correlation between the hypermethylation of the GSTP1 promoter region and histological grade of the tumor (p < 0.01), Nottingham Prognostic Index (p < 0.01) and lymph node metastasis (p < 0.05). Our results indicate that the GSTP1 hypermethylation is putative prognostic factor in breast cancer but a confirmation of its prognostic value is desirable in larger studies.<br /> Keywords: breast cancer, prognostic factors, hypermethylation, GSTP1, methylation-specific PCR

ISO 690:

Lasabova, Z., Tilandyova, P., Kajo, K., Zubor, P., Burjanivova,  ., Danko, J., Plank, L. 2010. Hypermethylation of the GSTP1 promoter region in breast cancer is associated with prognostic clinicopathological parameters. In NEOPLASMA, vol. 57, no.1, pp. 35-40. 0028-2685.

APA:

Lasabova, Z., Tilandyova, P., Kajo, K., Zubor, P., Burjanivova,  ., Danko, J., Plank, L. (2010). Hypermethylation of the GSTP1 promoter region in breast cancer is associated with prognostic clinicopathological parameters. NEOPLASMA, 57(1), 35-40. 0028-2685.