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A side population of cells from a human pancreatic carcinoma cell line harbors cancer stem cell characteristics

In: NEOPLASMA, vol. 56, no. 5
Y Wang - F Li - B Luo - X Wang - H Sun - S Liu - Y Cui - X Xu
Detaily:
Rok, strany: 2009, 371 - 378
O článku:
We used flow cytometry and a DNA-binding dye efflux assay to isolate a side population (SP) of cells with stem cell characteristics from the human pancreatic carcinoma cell line, PANC-1. Non-obese diabetic/severe combined immunodeficiency mouse xenograft experiments showed that SP cells were enriched in tumor initiating capability compared with non-SP cells. Cultured SP cells were able to differentiate into daughter cells and non-SP cells, through asymmetric division. Our study demonstrated that SP cells had high drug-resistance, both in vivo and in vitro. SP cells also showed significantly higher levels of mRNA expression for CD133, ABCG2 and Notch1, when compared to non-SP cells. Furthermore, xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133+ cells than untreated ones. We therefore suggest that these SP cells from the PANC-1 cell line were enriched with cancer stem cells. Keywords: Cancer stem cell, PANC1 cell, side population (SP), CD133, ABCG2
Ako citovať:
ISO 690:
Wang, Y., Li, F., Luo, B., Wang, X., Sun, H., Liu, S., Cui, Y., Xu, X. 2009. A side population of cells from a human pancreatic carcinoma cell line harbors cancer stem cell characteristics. In NEOPLASMA, vol. 56, no.5, pp. 371-378. 0028-2685.

APA:
Wang, Y., Li, F., Luo, B., Wang, X., Sun, H., Liu, S., Cui, Y., Xu, X. (2009). A side population of cells from a human pancreatic carcinoma cell line harbors cancer stem cell characteristics. NEOPLASMA, 56(5), 371-378. 0028-2685.