Facebook Instagram Twitter RSS Feed PodBean Back to top on side

Genetic analysis of KRAS mutation status in metastatic colorectal cancer patients

In: NEOPLASMA, vol. 56, no. 3
K Zavodna - M Konecny - T Krivulcik - S Spanik - R Behulova - M Vizvaryova - E Weismanova - S Galbavy - J Kausitz
Detaily:
Rok, strany: 2009, 275 - 278
O článku:
Colorectal carcinoma (CRC) represents a serious problem worldwide: in the Slovak republic are diagnosed about 2600 new CRC cases annually and its incidence is increasing. Colorectal cancer patients may succumb to the disease because of local recurrence or local formation of metastasis. Therefore, it is necessary to modulate therapeutic algorithm with new methods, leading to early diagnostic of CRC or changing the existing therapeutic procedures. <p align="justify">Recent progresses have been made in understanding of EGFR pathway involved in CRC carcinogenesis, especially the role of Ras protein. Mutations in <em>KRAS</em> oncogene are frequently found in human cancers, particularly colorectal, pancreatic, billiary tract and lung tumors. The presence of the <em>KRAS</em> mutations in metastatic colorectal cancer patients correlates with lack of response to the certain epidemal growth factor receptor (EGFR) inhibitor therapies, such as Panitumumab and Cetuximab. Consequently, screening for <em>KRAS</em> mutations status may be used as a prognostic marker, because the CRC patients with <em>KRAS</em> positive tumors have a worse prognosis.</p><p align="justify">The aim of our study was to establish the methods for rapid and sensitive detection of <em>KRAS</em> mutation status in formalin fixed paraffin embedded (FFPE) tissues DNA. We applied Real Time PCR analysis (TheraScreen KRAS Mutation Test Kit) and sequencing analysis (optimised for the analysis of FFPE tissues) to detect somatic mutations in codon 12 and 13 of <em>KRAS</em> gene. Both methods were used concurrently in the panel of DNA isolated from 25 colorectal FFPE tissues tumor. The positive or negative results from all 25 samples were identified by both methods independently. The <em>KRAS</em> mutations were presented in 8 of 25 patients (32%). Our results demonstrate that the Real Time PCR analysis can be used for detection of somatic <em>KRAS</em> mutations in FFPE clinical samples. However, we also recognize that the sequencing analysis of approximately 200bp amplicons may be used for <em>KRAS</em> mutations status screening, but with care of method sensitivity.</p> Keywords: KRAS mutation, genetic analysis, colorectal cancer, metastasis
Ako citovať:
ISO 690:
Zavodna, K., Konecny, M., Krivulcik, T., Spanik, S., Behulova, R., Vizvaryova, M., Weismanova, E., Galbavy, S., Kausitz, J. 2009. Genetic analysis of KRAS mutation status in metastatic colorectal cancer patients. In NEOPLASMA, vol. 56, no.3, pp. 275-278. 0028-2685.

APA:
Zavodna, K., Konecny, M., Krivulcik, T., Spanik, S., Behulova, R., Vizvaryova, M., Weismanova, E., Galbavy, S., Kausitz, J. (2009). Genetic analysis of KRAS mutation status in metastatic colorectal cancer patients. NEOPLASMA, 56(3), 275-278. 0028-2685.