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Administration of isothiocyanate (E-4IB) and cisplatin leads to altered signalling and lysosomal export in human ovarian carcinoma sensitive- and cisplatin-resistant cells

In: NEOPLASMA, vol. 56, no. 3
J Duraj - L Hunakova - J Bodo - J Jakubikova - J Chovancova - J Sedlak
Detaily:
Rok, strany: 2009, 208 - 214
O článku:
The aim of this study was to compare the effect of a new synthetic isothiocyanate derivative, ethyl 4-isothiocyanatobutanoate (E-4IB) and cisplatin (CDDP) in CDDP-sensitive human ovarian carcinoma cell line (A2780) and its resistant subline (A2780/CP). In parental cells, in comparison to untreated cells, sequential administration of both compounds led to higher exosomal dye (LysoTracker Green DND-26) retention and to alterations of mitogen-activated protein kinases (MAPKs), JNK, ERK and p38, or Akt kinase accompanied by changes in several anti- and pro-apoptotic molecules and lysosomal protein LAMP-1, as detected by Western blotting. On the contrary, variant A2780/CP cells were resistant to CDDP- or to combined sensitizer (E-4IB)/inducer (CDDP)-related apoptosis induction and exerted minor changes in the levels of these molecules. Keywords: ovarian carcinoma cells, isothiocyanate E-4IB, cisplatin (CDDP), cisplatin (CDDP) resistance, apoptosis, lysosomes/exosomes
Ako citovať:
ISO 690:
Duraj, J., Hunakova, L., Bodo, J., Jakubikova, J., Chovancova, J., Sedlak, J. 2009. Administration of isothiocyanate (E-4IB) and cisplatin leads to altered signalling and lysosomal export in human ovarian carcinoma sensitive- and cisplatin-resistant cells. In NEOPLASMA, vol. 56, no.3, pp. 208-214. 0028-2685.

APA:
Duraj, J., Hunakova, L., Bodo, J., Jakubikova, J., Chovancova, J., Sedlak, J. (2009). Administration of isothiocyanate (E-4IB) and cisplatin leads to altered signalling and lysosomal export in human ovarian carcinoma sensitive- and cisplatin-resistant cells. NEOPLASMA, 56(3), 208-214. 0028-2685.