In: NEOPLASMA, vol. 55, no. 6
J. Lakota - L. Skultety - M. Dubrovcakova - Č. Altaner
Detaily:
Rok, strany: 2008, 488 - 492
O článku:
Here we report patients with Hodgkin’s disease and multiple myeloma, who relapsed/progressed after high dose therapy
and autologous stem cell transplantation. In patients who developed aplastic anemia type syndrome, spontaneous tumor
regression was observed and concomitantly high titers of serum autoantibodies were found. In order to identify the antibody
specificity, two-dimensional electrophoresis, blotting and immunoreactions were used to analyze the peripheral blood stem
cell extract with autoantibodies containing serum. The unique protein spot visualized exclusively by serum of patients with
aplastic anemia type syndrome was identified as carbonic anhydrase I (CA I, accession No. P00915 and Q7M316) by means
of mass spectrometry. The specificity of autoantibodies was confirmed by reaction with commercial CAs I, II, IX and XII.
Immunoreaction in Western blots with these CA isoforms differed in sera obtained from patients with various types of the
disease. Sera of Hodgkin’s disease patients reacted with CA I, II and XII; sera of multiple myeloma patients reacted with the
CA I, II, XII and IX. Patients developing and/or possessing CA autoantibodies had a significant survival benefit over those
who did not develop CA anhydrase autoantibodies. Possible relevance of the presence of CA autoantibodies and clinical
outcome is discussed.
Key words: stem cell transplantation, spontaneous remission, serum autoantibodies, carbonic anhydrases.
Ako citovať:
ISO 690:
Lakota, J., Skultety, L., Dubrovcakova, M., Altaner, Č. 2008. Presence of serum carbonic anhydrase autoantibodies in patients relapsed
after autologous stem cell transplantation indicates an improved prognosis. In NEOPLASMA, vol. 55, no.6, pp. 488-492. 0028-2685.
APA:
Lakota, J., Skultety, L., Dubrovcakova, M., Altaner, Č. (2008). Presence of serum carbonic anhydrase autoantibodies in patients relapsed
after autologous stem cell transplantation indicates an improved prognosis. NEOPLASMA, 55(6), 488-492. 0028-2685.