In: NEOPLASMA, vol. 55, no. 6
A. Perhavec - P. Cerkovnik - S. Novakovic - J. Žgajnar
Detaily:
Rok, strany: 2008, 549 - 554
O článku:
One of the most important changes, which make cancer cells immortal, is reactivation of the telomerase enzyme. Human
telomerase is composed of at least two subunits, hTERT and hTR. Many investigators have already detected telomerase
mRNA in bodily fluids. The first aim of our study was to find out if there is a difference in the appearance frequency of
detectable hTERT mRNA in plasma of early breast cancer patients, non-cancer patients and healthy individuals. The second
aim was to determine whether surgical removal of the tumor affects the presence of hTERT mRNA in plasma of early breast
cancer patients.
87 patients with early breast cancer, 22 non-cancer patients and 21 healthy individuals were included in the study. From
early breast cancer patients, two blood samples were collected, the first prior and the second 24 hours after the surgical
removal of the tumor. From other individuals one blood sample was collected. The presence or absence of hTERT mRNA
was determined from all blood samples.
47% of early breast cancer patients, 32% of non-cancer patients and 5% of healthy individuals tested positive for the
presence of hTERT mRNA in plasma. The difference between early breast cancer patients and healthy individuals was
statistically significant (p<0,001). Among early breast cancer patients, 26% were positive for the presence of plasma hTERT
mRNA before and after the surgical removal of the tumor, 21% were positive before and negative after, 36% were negative
before and after and 17% were negative before and positive after the surgical removal of the tumor.
In conclusion, we found statistically significant difference of hTERT mRNA presence in plasma of early breast cancer
patients when compared to healthy individuals. Second, we found that hTERT mRNA in plasma of early breast cancer
patients is affected by the surgical removal of the tumor.
Key words: Breast cancer, breast surgery, telomerase, hTERT mRNA
Ako citovať:
ISO 690:
Perhavec, A., Cerkovnik, P., Novakovic, S., Žgajnar, J. 2008. The hTERT mRNA in plasma samples of early breast cancer patients,
non-cancer patients and healthy individuals. In NEOPLASMA, vol. 55, no.6, pp. 549-554. 0028-2685.
APA:
Perhavec, A., Cerkovnik, P., Novakovic, S., Žgajnar, J. (2008). The hTERT mRNA in plasma samples of early breast cancer patients,
non-cancer patients and healthy individuals. NEOPLASMA, 55(6), 549-554. 0028-2685.