Combined molecular biological and molecular cytogenetic analysis of genomic changes in 146 patients with B-cell chronic lymphocytic leukemia

Rok, strany: 2008, 400 - 408

Interphase fluorescence in situ hybridization was used to detect common deletions in B-CLL patients as well as trisomy 12 and aberrations of IgH gene complex at 14q32.33 where we evaluated not only translocation-like signal pattern but also deletions. 120 (82%) patients showed genetic changes – del(13)(q14) 95 (62%), deletion of ATM gene 22 (15%), deletion of p53 gene 25 (17%) and trisomy 12 was proved in 18 (12%) cases. IgH rearrangements were detected in 45 (31%), split of the signals in 11 (8%), deletion of 3´segment flanking IgH gene in 5 (3%) and deletions of variable segment in 29 (20%) patients. Although deletions of 3´segment flanking IgH gene complex are supposed to have an adverse prognostic impact and the genetic background of variable segment deletions is believed to be most probably physiological, we assumed a detailed mapping of the 14q32.33 region will be needed to unravel these mysteries. Key words: B-CLL; I-FISH; IgH gene complex deletions; prognosis

ISO 690:

Berkova, A., Pavlištová, L., Babicka, L., Houskova, L., Tajtlova, J., Balazi, P., Cmunt, E., Schwarz, J., Karban, J., Trneny, M., Březinová, J., Zemanova, Z., Michalova, K. 2008. Combined molecular biological and molecular cytogenetic analysis of genomic changes in 146 patients with B-cell chronic lymphocytic leukemia. In NEOPLASMA, vol. 55, no.5, pp. 400-408. 0028-2685.

APA:

Berkova, A., Pavlištová, L., Babicka, L., Houskova, L., Tajtlova, J., Balazi, P., Cmunt, E., Schwarz, J., Karban, J., Trneny, M., Březinová, J., Zemanova, Z., Michalova, K. (2008). Combined molecular biological and molecular cytogenetic analysis of genomic changes in 146 patients with B-cell chronic lymphocytic leukemia. NEOPLASMA, 55(5), 400-408. 0028-2685.